LOCUS       sequence-142042-         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flag tagged human MFF isoform 4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Toyama EQ, Herzig S, Courchet J, Lewis TL Jr, Loson OC, Hellberg K, 
            Young NP, Chen H, Polleux F, Chan DC, Shaw RJ
  TITLE     Metabolism. AMP-activated protein kinase mediates mitochondrial 
            fission in response to energy stress.
  JOURNAL   Science. 2016 Jan 15;351(6270):275-81. doi: 10.1126/science.aab4138.
  PUBMED    26816379
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-01-15- 15"; volume: "351"; 
            issue: "6270"; pages: "275-81"
COMMENT     Sequence Label: pcDNA3 Flag MFF iso4 WT
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    98..122
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             131..154
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(822..846)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             860..901
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             920..937
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
ORIGIN
        1 ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agttaagctg agcatcaaca agtttgtaca aaaaagcagg
      121 cttcaccatg gattacaagg acgacgatga caagggcgga tctatggcag aaattagtcg
      181 aattcagtac gaaatggaat atactgaagg cattagtcag cgaatgaggg tcccagaaaa
      241 gttaaaagta gcaccgccaa acgctgacct ggaacaagga ttccaagaag gagttccaaa
      301 tgctagtgtg ataatgcaag ttccggagag gattgttgta gcaggaaata atgaagatgt
      361 ttcattttca agaccagcag atcttgacct tattcagtca actcccttta aacccctggc
      421 actgaaaaca ccacctcgtg tacttacgct gagtgaaaga ccactagatt ttctggattt
      481 agaaagacct cctacaaccc ctcaaaatga agaaatccga gcagttggca gactaaaaag
      541 agagcggtct atgagtgaaa atgctgttcg ccaaaatgga cagctggtca gaaatgattc
      601 tctgtatggc atttcaaata tagatacaac cattgaagga acgtcagatg acctgactgt
      661 tgtagatgca gcttcactaa gacgacagat aatcaaacta aatagacgtc tacaacttct
      721 ggaagaggag aacaaagaac gtgctaaaag agaaatggtc atgtattcaa ttactgtagc
      781 tttctggctg cttaatagct ggctctggtt tcgccgctag gacccagctt tcttgtacaa
      841 agtggttgat gctgttaacg ggaagcctat ccctaaccct ctcctcggtc tcgattctac
      901 gcgtaccggt gctggtggct gttgtcctgg ctgttgcggt ggcggctagt aatgagttta
      961 aacgggg
//
