LOCUS       sequence-142046-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Zfhx3. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Zfhx3
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(600..618)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(601..618)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(636..652)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(636..652)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(649..671)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    660..676
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(684..690,691..708,709..714))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    729..750
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gccgccatgg cggccgcggg aattcgattc agcttcttct ggtgtttgat gaggtcaaag
       61 atgcgctgaa acacgaggct gcactttttg cactggtaat tcaagttgct cgtccgaatg
      121 tagcgatcgt tggtaagctc gcgccgctct ccgtctttgc cctccccctg attctcgtaa
      181 ttcttcctgg ctttctgtcg ggcgttctga aaccacacca ctatgacacg ggttggaagg
      241 ttcagtaagt tagaaagctg ctcaaattca tcatcctttg ggtaagcatt ggcatcaaag
      301 aagtcttgca ggactcggag ctggtagtcg gtaaaccttg ttctcgagga ccgcttgctc
      361 ccccagtact cctgcttctg gggttcgggg gaagggggcc gggagtcgat cttgagttcc
      421 tccagactgg tgatgggagg gttgctgaag ttgtacgggg agtccttgtt ccgttgccgc
      481 tccttgaata gagtgtttct gaaccagtgc ttgatcacta atcactagtg aattcgcggc
      541 cgcctgcagg tcgaccatat gggagagctc ccaacgcgtt ggatgcatag cttgagtatt
      601 ctatagtgtc acctaaatag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
      661 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
      721 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
      781 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcgg
//
