LOCUS       sequence-142060-         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Zhx3. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Zhx3
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(703..721)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(704..721)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(739..755)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(739..755)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(752..774)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    763..779
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(787..793,794..811,812..817))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    832..853
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggcggccgcg ggaattcgat ttgaaggtaa cagtgggctc tttgtcaatc agaacacaag
       61 acaagtctgg cacaaaatgt ggcttgcctc cactcagtga tgcagagatc tcttccaaca
      121 atctgttccc ttgagaagac aagtagggcc aggcctggtg cagttctgag catgcctggg
      181 actcccactg ttgcagagga tgagggtccc tgtggtctca gcttcccaga aaacagacat
      241 ggcattgtca gagctgtact gttttggtaa cccgagttcc tatgtattga aaatgtctgt
      301 agtagtatgc tttattaaat gaatataggc agctgtatat gctaacaact tgattctggg
      361 tctaggtata tgcaatctga attgtccctg ggaggccatg gaaaggtcct gctctgtggg
      421 aagatgggct gatgagaaac cattgctatt tgctcagaaa tgtcaggcgg aaggtggttg
      481 gggcggtggc aggttctggg ttcagcgagg ctccagaggc tctcctggtg cctggcagct
      541 ggccatcaag atgatatgtg gcagttttgg cccttccaca tggcatacag catcccagac
      601 tggccggtcc ttcacagtaa tcaatcacta gtgaattcgc ggccgcctgc aggtcgacca
      661 tatgggagag ctcccaacgc gttggatgca tagcttgagt attctatagt gtcacctaaa
      721 tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
      781 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
      841 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
      901 ccagctgcat taatgaatcg gccaacgcgc ggggaaaagc
//
