LOCUS       sequence-142066-        1866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression plasmid encoding wild type human BRD9 with a C
            terminal 3x FLAG-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Hohmann AF, Martin LJ, Minder JL, Roe JS, Shi J, Steurer S, Bader G,
            McConnell D, Pearson M, Gerstberger T, Gottschamel T, Thompson D, 
            Suzuki Y, Koegl M, Vakoc CR
  TITLE     Sensitivity and engineered resistance of myeloid leukemia cells to 
            BRD9 inhibition.
  JOURNAL   Nat Chem Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115.
  PUBMED    27376689
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115."
COMMENT     Sequence Label: MSCV-Cterm_3xFL_BRD9-PGK-Puro-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..1866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1296..1307
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             1798..1863
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atgggcaaga agcacaagaa gcacaaggcc gagtggcgct cgtcctacga ggattatgcc
       61 gacaagcccc tggagaagcc tctaaagcta gtcctgaagg tcggaggaag tgaagtgact
      121 gaactctcag gatccggcca cgactccagt tactatgatg acaggtcaga ccatgagcga
      181 gagaggcaca aagaaaagaa aaagaagaag aagaagaagt ccgagaagga gaagcatctg
      241 gacgatgagg aaagaaggaa gcgaaaggaa gagaagaagc ggaagcgaga gagggagcac
      301 tgtgacacgg agggagaggc tgacgacttt gatcctggga agaaggtgga ggtggagccg
      361 cccccagatc ggccagtccg agcgtgccgg acacagccag ccgaaaatga gagcacacct
      421 attcagcaac tcctggaaca cttcctccgc cagcttcaga gaaaagatcc ccatggattt
      481 tttgcttttc ctgtcacgga tgcaattgct cctggatatt caatgataat aaaacatccc
      541 atggattttg gcaccatgaa agacaaaatt gtagctaatg aatacaagtc agttacggaa
      601 tttaaggcag atttcaagct gatgtgtgat aatgcaatga catacaatag gccagatacc
      661 gtgtactaca agttggcgaa gaagatcctt cacgcaggct ttaagatgat gagcaaacag
      721 gcagctcttt tgggcaatga agatacagct gttgaggaac ctgtccctga agttgtacca
      781 gtacaagtag aaactgccaa gaaatccaaa aagccgagta gagaagttat cagctgcatg
      841 tttgagcctg aagggaatgc ctgcagcttg acggacagta ccgcagagga gcacgtgctg
      901 gcgctggtgg agcacgcagc tgacgaagct cgggacagga tcaaccggtt cctcccaggc
      961 ggcaagatgg gctatctgaa gaggaacggg gacgggagcc tgctctacag cgtggtcaac
     1021 acggccgagc cggacgctga tgaggaggag acccacccgg tggacttgag ctcgctctcc
     1081 agtaagctac tcccaggctt caccacgctg ggcttcaaag acgagagaag aaacaaagtc
     1141 acctttctct ccagtgccac tactgcgctt tcgatgcaga ataattcagt atttggcgac
     1201 ttgaagtcgg acgagatgga gctgctctac tcagcctacg gagatgagac aggcgtgcag
     1261 tgtgcgctga gcctgcagga gtttgtgaag gatgctggga gctacagcaa gaaagtggtg
     1321 gacgacctcc tggaccagat cacaggcgga gaccactcta ggacgctctt ccagctgaag
     1381 cagagaagaa atgttcccat gaagcctcca gatgaagcca aggttgggga caccctagga
     1441 gacagcagca gctctgttct ggagttcatg tcgatgaagt cctatcccga cgtttctgtg
     1501 gatatctcca tgctcagctc tctggggaag gtgaagaagg agctggaccc tgacgacagc
     1561 catttgaact tggatgagac gacgaagctc ctgcaggacc tgcacgaagc acaggcggag
     1621 cgcggcggct ctcggccgtc gtccaacctc agctccctgt ccaacgcctc cgagagggac
     1681 cagcaccacc tgggaagccc ttctcgcctg agtgtcgggg agcagccaga cgtcacccac
     1741 gacccctatg agtttcttca gtctccagag cctgcggcct ctgccaagac cggaggcgat
     1801 tacaaagacc atgacggtga ttataaagat catgatatcg attacaagga tgacgatgac
     1861 aagtga
//
