LOCUS       sequence-142082-         607 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gRNA_B to knockout human AMPK alpha 1 using Cas9n.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Toyama EQ, Herzig S, Courchet J, Lewis TL Jr, Loson OC, Hellberg K, 
            Young NP, Chen H, Polleux F, Chan DC, Shaw RJ
  TITLE     Metabolism. AMP-activated protein kinase mediates mitochondrial 
            fission in response to energy stress.
  JOURNAL   Science. 2016 Jan 15;351(6270):275-81. doi: 10.1126/science.aab4138.
  PUBMED    26816379
REFERENCE   2  (bases 1 to 607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-01-15- 15"; volume: "351"; 
            issue: "6270"; pages: "275-81"
COMMENT     Sequence Label: pX462-hPRKAA1-gRNA_B
FEATURES             Location/Qualifiers
     source          1..607
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(313..333)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(340..405)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      405..414
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agagctgtcg tccaccttgg ccatctcgtt gctgaagatc tcttgcagat agcagatccg
       61 gttcttccgt ctggtgtatc ttcttctggc ggttctcttc agccgggtgg cctcggctgt
      121 ttcgccgctg tcgaacagca gggctccgat caggttcttc ttgatgctgt gccggtcggt
      181 gttgcccagc accttgaatt tcttgctggg caccttgtac tcgtcggtga tcacggccca
      241 gcccacagag ttggtgccga tggccaggcc gatgctgtac ttcttgtcgg ctgctgggac
      301 tccgtggata ccgaccttcc gcttcttctt tggggccatc ttatcgtcat cgtctttgta
      361 atcaatatca tgatccttgt agtctccgtc gtggtcctta tagtccatgg tggcaccggt
      421 ccaacctgaa aaaaagtgat ttcaggcagg tgctccaggt aattaaacat taatacccca
      481 ccaaccaacc atcccttaaa cccttacctc ttgctcagct aattacagcc cggaggagaa
      541 gggccgtccc gcccgctcac ctgtgggagt aacgcggtca gtcagagccg gggcgggcgg
      601 cgcgagg
//
