LOCUS       sequence-142088-         530 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gRNA_B to knockout human AMPK alpha 2 using Cas9n.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Toyama EQ, Herzig S, Courchet J, Lewis TL Jr, Loson OC, Hellberg K, 
            Young NP, Chen H, Polleux F, Chan DC, Shaw RJ
  TITLE     Metabolism. AMP-activated protein kinase mediates mitochondrial 
            fission in response to energy stress.
  JOURNAL   Science. 2016 Jan 15;351(6270):275-81. doi: 10.1126/science.aab4138.
  PUBMED    26816379
REFERENCE   2  (bases 1 to 530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-01-15- 15"; volume: "351"; 
            issue: "6270"; pages: "275-81"
COMMENT     Sequence Label: pX462-hPRKAA2-gRNA_B
FEATURES             Location/Qualifiers
     source          1..530
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(317..337)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(344..409)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      409..418
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtggagagct gtcgtccacc ttggccatct cgttgctgaa gatctcttgc agatagcaga
       61 tccggttctt ccgtctggtg tatcttcttc tggcggttct cttcagccgg gtggcctcgg
      121 ctgtttcgcc gctgtcgaac agcagggctc cgatcaggtt cttcttgatg ctgtgccggt
      181 cggtgttgcc cagcaccttg aatttcttgc tgggcacctt gtactcgtcg gtgatcacgg
      241 cccagcccac agagttggtg ccgatggcca ggccgatgct gtacttcttg tcggctgctg
      301 ggactccgtg gataccgacc ttccgcttct tctttggggc catcttatcg tcatcgtctt
      361 tgtaatcaat atcatgatcc ttgtagtctc cgtcgtggtc cttatagtcc atggtggcac
      421 cggtccaacc tgaaaaaaag tgatttcagg caggtgctcc aggtaattaa acattaatac
      481 cccaccaacc aaccatccct taaaccctta cctcttgctc agctaattac
//
