LOCUS       sequence-142099-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector for His(6) tagged Formylglycine Generating Enzyme 
            (FGE) in bacterial host..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Liu J, Hanne J, Britton BM, Shoffner M, Albers AE, Bennett J, 
            Zatezalo R, Barfield R, Rabuka D, Lee JB, Fishel R
  TITLE     An Efficient Site-Specific Method for Irreversible Covalent Labeling
            of Proteins with a Fluorophore.
  JOURNAL   Sci Rep. 2015 Nov 19;5:16883. doi: 10.1038/srep16883.
  PUBMED    26582263
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-11-19"; volume: "5:16883. doi"; pages: " 
            10.1038/srep16883"
COMMENT     Sequence Label: His-FGE
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggtgccgc gcggcagcca tatgctgacc gagttggttg acctgcccgg cggatcgttc
      121 cgcatgggct cgacgcgctt ctaccccgaa gaagcgccga ttcataccgt gaccgtgcgc
      181 gcctttgcgg tagagcgaca cccggtgacc aacgcgcaat ttgccgaatt cgtctccgcg
      241 acaggctatg tgacggttgc agaacaaccc cttgaccccg ggctctaccc aggagtggac
      301 gcagcagacc tgtgtcccgg tgcgatggtg ttttgtccga cggccgggcc ggtcgacctg
      361 cgtgactggc ggcaatggtg ggactgggta cctggcgcct gctggcgcca tccgtttggc
      421 cgggacagcg atatcgccga ccgagccggc cacccggtcg tacaggtggc ctatccggac
      481 gccgtggcct acgcacgatg ggctggtcga cgcctaccga ccgaggccga gtgggagtac
      541 gcggcccgtg gcggaaccac ggcaacctat gcgtggggcg accaggagaa gccggggggc
      601 atgctcatgg cgaacacctg gcagggccgg tttccttacc gcaacgacgg tgcattgggc
      661 tgggtgggaa cctccccggt gggcaggttt ccggccaacg ggtttggctt gctcgacatg
      721 atcggaaacg tttgggagtg gaccaccacc gagttctatc cacaccatcg catcgatcca
      781 ccctcgacgg cctgctgcgc accggtcaag ctcgctacag ccgccgaccc gacgatcagc
      841 cagaccctca agggcggctc gcacctgtgc gcgccggagt actgccaccg ctaccgcccg
      901 gcggcgcgct cgccgcagtc gcaggacacc gcgaccaccc atatcgggtt ccggtgcgtg
      961 gccgacccgg tgtccgggta gctcgaggat ccggctgcta acaaagcccg aaag
//
