LOCUS       sequence-142121-        9179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses three enzymes to convert phenylpropanoic acids to 
            flavanones in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9179)
  AUTHORS   Jones JA, Vernacchio VR, Sinkoe AL, Collins SM, Ibrahim MH, Lachance
            DM, Hahn J, Koffas MA
  TITLE     Experimental and computational optimization of an Escherichia coli 
            co-culture for the efficient production of flavonoids.
  JOURNAL   Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006.
  PUBMED    26860871
REFERENCE   2  (bases 1 to 9179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2016-02-6"; volume: "35:55-63. doi"; pages: " 
            10.1016/j.ymben.2016.01.006"
COMMENT     Sequence Label: pETM6-At4CL-PhCHS-MsCHI
FEATURES             Location/Qualifiers
     source          1..9179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1770..1814
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      1867..1914
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        1948..1966
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1967..1991
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2006..2028
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3227..3271
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      3324..3371
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3405..3423
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3424..3448
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3463..3485
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      4211..4258
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4302..4757
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     4599..4620
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(4872..5663,5664..5732))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5495..5514
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5733..5825)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5906..6494
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6395..6414
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6648..6665
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6680..6822
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6748..6767)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6908..6930
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6924..7115)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7687..8769)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8731..8750
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8770..8847)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9053..9072
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(9163..9179,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatggcgcca caagaacaag cagtttctca ggtgatggag aaacagagca
      121 acaacaacaa cagtgacgtc attttccgat caaagttacc ggatatttac atcccgaacc
      181 acctatctct ccacgactac atcttccaaa acatctccga attcgccact aagccttgcc
      241 taatcaacgg accaaccggc cacgtgtaca cttactccga cgtccacgtc atctcccgcc
      301 aaatcgccgc caattttcac aaactcggcg ttaaccaaaa cgacgtcgtc atgctcctcc
      361 tcccaaactg tcccgaattc gtcctctctt tcctcgccgc ctccttccgc ggcgcaaccg
      421 ccaccgccgc aaaccctttc ttcactccgg cggagatagc taaacaagcc aaagcctcca
      481 acaccaaact cataatcacc gaagctcgtt acgtcgacaa aatcaaacca cttcaaaacg
      541 acgacggagt agtcatcgtc tgcatcgacg acaacgaatc cgtgccaatc cctgaaggct
      601 gcctccgctt caccgagttg actcagtcga caaccgaggc atcagaagtc atcgactcgg
      661 tggagatttc accggacgac gtggtggcac taccttactc ctctggcacg acgggattac
      721 caaaaggagt gatgctgact cacaagggac tagtcacgag cgttgctcag caagtcgacg
      781 gcgagaaccc gaatctttat ttccacagcg atgacgtcat actctgtgtt ttgcccatgt
      841 ttcatatcta cgctttgaac tcgatcatgt tgtgtggtct tagagttggt gcggcgattc
      901 tgataatgcc gaagtttgag atcaatctgc tattggagct gatccagagg tgtaaagtga
      961 cggtggctcc gatggttccg ccgattgtgt tggccattgc gaagtcttcg gagacggaga
     1021 agtatgattt gagctcgata agagtggtga aatctggtgc tgctcctctt ggtaaagaac
     1081 ttgaagatgc cgttaatgcc aagtttccta atgccaaact cggtcaggga tacggaatga
     1141 cggaagcagg tccagtgctc gcaatgtcgt taggttttgc aaaggaacct tttccggtta
     1201 agtcaggagc ttgtggtact gttgtaagaa atgctgagat gaaaatagtt gatccagaca
     1261 ccggagattc tctttcgagg aatcaacccg gtgagatttg tattcgtggt caccagatca
     1321 tgaaaggtta cctcaacaat ccggcagcta cagcagagac cattgataaa gacggttggc
     1381 ttcatactgg agatattgga ttgatcgatg acgatgacga gcttttcatc gttgatcgat
     1441 tgaaagaact tatcaagtat aaaggttttc aggtagctcc ggctgagcta gaggctttgc
     1501 tcatcggtca tcctgacatt actgatgttg ctgttgtcgc aatgaaagaa gaagcagctg
     1561 gtgaagttcc tgttgcattt gtggtgaaat cgaaggattc ggagttatca gaagatgatg
     1621 tgaagcaatt cgtgtcgaaa caggttgtgt tttacaagag aatcaacaaa gtgttcttca
     1681 ctgaatccat tcctaaagct ccatcaggga agatattgag gaaagatctg agggcaaaac
     1741 tagcaaatgg attgtgactc gagtctggta aagaaaccgc tgctgcgaaa tttgaacgcc
     1801 agcacatgga ctcgtctact agtcgcagct taattaacct aaactgctgc caccgctgag
     1861 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctagga
     1921 tcgagatcga tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg
     1981 ataacaattc ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatggt
     2041 gacagtcgag gagtatcgta aggcacaacg tgctgaaggt ccagccactg tcatggccat
     2101 tggaacagcc acaccttcaa actgtgttga tcaaagcact taccctgatt tttattttcg
     2161 tatcactaac agtgagcaca agactgatct taaggagaaa tttaagcgca tgtgtgaaaa
     2221 atcaatgatt aagaaaaggt acatgcactt aacagaggaa atcttgaaag agaatcctag
     2281 tatgtgtgaa tacatggcac cttctcttga tgctaggcaa gacatagtgg tggttgaagt
     2341 gcccaaactt ggcaaagagg cagctcaaaa ggccatcaag gaatggggcc agcccaagtc
     2401 caaaattacc catttggtct tttgcacaac cagtggtgtg gacatgcctg ggtgtgacta
     2461 tcaactcact aagctacttg ggcttcgtcc atcggtcaag aggcttatga tgtaccaaca
     2521 aggttgcttt gctggtggca cggttcttcg gttagccaag gacttggctg aaaacaacaa
     2581 gggcgctcga gtccttgttg tttgttcaga aatcaccgcg gtcactttcc gtgggccaaa
     2641 tgatactcat ttggatagtt tagttggcca agcacttttt ggtgatgggg caggcgcgat
     2701 cattataggt tctgatccaa ttccaggggt cgaaaggcct ttgttcgagc tcgtttcagc
     2761 agcccaaact cttctcccag atagccatgg tgctattgat ggccatctcc gtgaagttgg
     2821 gcttacattc cacttactca aagatgttcc tgggctgatc tcaaaaaata ttgagaagag
     2881 ccttgaggaa gcattcaaac ctttgggcat ttctgattgg aactctctat tctggattgc
     2941 tcatccaggt gggcctgcaa ttttggacca agttgaaata aagttgggcc taaagcccga
     3001 gaaacttaag gctacaagga atgtgttaag taactatggt aacatgtcaa gtgcttgtgt
     3061 actgtttatt ttggatgaaa tgagaaaggc ctcagccaaa gaaggtttag gaactactgg
     3121 tgaagggctt gagtggggtg ttctttttgg atttgggcct gggctaacag ttgagactgt
     3181 tgtcctccac agtgttgcta cttaataggg taccctcgag tctggtaaag aaaccgctgc
     3241 tgcgaaattt gaacgccagc acatggactc gtctactagt cgcagcttaa ttaacctaaa
     3301 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
     3361 ggggtttttt gctaggatcg agatcgatct cgatcccgcg aaattaatac gactcactat
     3421 aggggaattg tgagcggata acaattcccc tctagaaata attttgttta actttaagaa
     3481 ggagatatac atatggctgc atcaatcacc gcaatcactg tggagaacct tgaataccca
     3541 gcggtggtta cctctccggt caccggcaaa tcatatttcc tcggtggcgc tggggagaga
     3601 ggattgacca ttgaaggaaa cttcatcaag ttcactgcca taggtgttta tttggaagat
     3661 atagcagtgg cttcactagc tgccaaatgg aagggtaaat catctgaaga gttacttgag
     3721 acccttgact tttacagaga catcatctca ggtccctttg aaaagttaat tagagggtca
     3781 aagattaggg aattgagtgg tcctgagtac tcaaggaagg ttatggagaa ctgtgtggca
     3841 cacttgaaat cagttggaac ttatggagat gcagaagctg aagctatgca aaaatttgct
     3901 gaagctttca agcctgttaa ttttccacct ggtgcctctg ttttctacag gcaatcacct
     3961 aatggaatat tagggcttag tttctctccg gatacaagta taccagaaaa ggaggctgca
     4021 ctcatagaga acaaggcagt ttcatcagca gtgttggaga ctatgatcgg cgagcacgct
     4081 gtttcccctg atcttaagcg ctgtttagct gcaagattac ctgcgttgtt gaacgagggt
     4141 gctttcaaga ttggaaactg aactagtcgc agcttaatta acctaaactg ctgccaccgc
     4201 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
     4261 agcgaaagga ggagtcgact atatccggat tggcgaatgg gacgcgccct gtagcggcgc
     4321 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4381 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4441 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4501 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4561 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4621 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     4681 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     4741 attaacgttt acaatttctg gcggcacgat ggcatgagat tatcaaaaag gatcttcacc
     4801 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     4861 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     4921 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     4981 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     5041 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     5101 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     5161 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     5221 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     5281 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     5341 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     5401 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     5461 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     5521 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     5581 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     5641 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     5701 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaatc atgattgaag
     5761 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     5821 acaaataggt catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     5881 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     5941 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6001 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     6061 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     6121 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     6181 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6241 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6301 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6361 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     6421 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     6481 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     6541 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     6601 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     6661 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     6721 cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     6781 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     6841 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     6901 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     6961 cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca
     7021 tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg
     7081 gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt
     7141 ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact
     7201 gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg
     7261 cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta
     7321 ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag
     7381 ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt
     7441 gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt
     7501 gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg
     7561 agcacgatca tgctagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct
     7621 ctcaagggca tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg
     7681 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     7741 ggccaacgcg cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac
     7801 cagtgagacg ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa
     7861 gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg
     7921 gatataacat gagctgtctt cggtatcgtc gtatcccact accgagatgt ccgcaccaac
     7981 gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac
     8041 cagcatcgca gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga
     8101 catggcactc cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata
     8161 tttatgccag ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag
     8221 cgcgatttgc tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc
     8281 atgggagaaa ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg
     8341 aacattagtg caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat
     8401 gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac
     8461 gccgcttcgt tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt
     8521 aatcgccgcg acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat
     8581 cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc
     8641 cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac
     8701 cacgcgggaa acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac
     8761 tggtttcaca ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg
     8821 aaaggttttg cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct
     8881 gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg
     8941 gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca
     9001 cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt
     9061 cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc
     9121 cggcgtagcc taggatcgag atcgatctcg atcccgcgaa attaatacga ctcactata
//
