LOCUS       sequence-142122-        9254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses three enzymes to convert phenylpropanoic acids to 
            flavanones in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9254)
  AUTHORS   Jones JA, Vernacchio VR, Sinkoe AL, Collins SM, Ibrahim MH, Lachance
            DM, Hahn J, Koffas MA
  TITLE     Experimental and computational optimization of an Escherichia coli 
            co-culture for the efficient production of flavonoids.
  JOURNAL   Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006.
  PUBMED    26860871
REFERENCE   2  (bases 1 to 9254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2016-02-6"; volume: "35:55-63. doi"; pages: " 
            10.1016/j.ymben.2016.01.006"
COMMENT     Sequence Label: pETM6-At4CL-CmCHS-MsCHI
FEATURES             Location/Qualifiers
     source          1..9254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1770..1814
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      1867..1914
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        1948..1966
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1967..1991
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2006..2028
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3302..3346
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      3399..3446
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3480..3498
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3499..3523
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3538..3560
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      4286..4333
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4377..4832
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     4674..4695
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(4947..5738,5739..5807))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5570..5589
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5808..5900)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5981..6569
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6470..6489
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6723..6740
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6755..6897
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6823..6842)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6983..7005
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6999..7190)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7762..8844)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8806..8825
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8845..8922)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9128..9147
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(9238..9254,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatggcgcca caagaacaag cagtttctca ggtgatggag aaacagagca
      121 acaacaacaa cagtgacgtc attttccgat caaagttacc ggatatttac atcccgaacc
      181 acctatctct ccacgactac atcttccaaa acatctccga attcgccact aagccttgcc
      241 taatcaacgg accaaccggc cacgtgtaca cttactccga cgtccacgtc atctcccgcc
      301 aaatcgccgc caattttcac aaactcggcg ttaaccaaaa cgacgtcgtc atgctcctcc
      361 tcccaaactg tcccgaattc gtcctctctt tcctcgccgc ctccttccgc ggcgcaaccg
      421 ccaccgccgc aaaccctttc ttcactccgg cggagatagc taaacaagcc aaagcctcca
      481 acaccaaact cataatcacc gaagctcgtt acgtcgacaa aatcaaacca cttcaaaacg
      541 acgacggagt agtcatcgtc tgcatcgacg acaacgaatc cgtgccaatc cctgaaggct
      601 gcctccgctt caccgagttg actcagtcga caaccgaggc atcagaagtc atcgactcgg
      661 tggagatttc accggacgac gtggtggcac taccttactc ctctggcacg acgggattac
      721 caaaaggagt gatgctgact cacaagggac tagtcacgag cgttgctcag caagtcgacg
      781 gcgagaaccc gaatctttat ttccacagcg atgacgtcat actctgtgtt ttgcccatgt
      841 ttcatatcta cgctttgaac tcgatcatgt tgtgtggtct tagagttggt gcggcgattc
      901 tgataatgcc gaagtttgag atcaatctgc tattggagct gatccagagg tgtaaagtga
      961 cggtggctcc gatggttccg ccgattgtgt tggccattgc gaagtcttcg gagacggaga
     1021 agtatgattt gagctcgata agagtggtga aatctggtgc tgctcctctt ggtaaagaac
     1081 ttgaagatgc cgttaatgcc aagtttccta atgccaaact cggtcaggga tacggaatga
     1141 cggaagcagg tccagtgctc gcaatgtcgt taggttttgc aaaggaacct tttccggtta
     1201 agtcaggagc ttgtggtact gttgtaagaa atgctgagat gaaaatagtt gatccagaca
     1261 ccggagattc tctttcgagg aatcaacccg gtgagatttg tattcgtggt caccagatca
     1321 tgaaaggtta cctcaacaat ccggcagcta cagcagagac cattgataaa gacggttggc
     1381 ttcatactgg agatattgga ttgatcgatg acgatgacga gcttttcatc gttgatcgat
     1441 tgaaagaact tatcaagtat aaaggttttc aggtagctcc ggctgagcta gaggctttgc
     1501 tcatcggtca tcctgacatt actgatgttg ctgttgtcgc aatgaaagaa gaagcagctg
     1561 gtgaagttcc tgttgcattt gtggtgaaat cgaaggattc ggagttatca gaagatgatg
     1621 tgaagcaatt cgtgtcgaaa caggttgtgt tttacaagag aatcaacaaa gtgttcttca
     1681 ctgaatccat tcctaaagct ccatcaggga agatattgag gaaagatctg agggcaaaac
     1741 tagcaaatgg attgtgactc gagtctggta aagaaaccgc tgctgcgaaa tttgaacgcc
     1801 agcacatgga ctcgtctact agtcgcagct taattaacct aaactgctgc caccgctgag
     1861 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctagga
     1921 tcgagatcga tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg
     1981 ataacaattc ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatggc
     2041 tacggtccaa gaaatccgca acgctcaacg cgcagatggt ccggcgacgg tcctggcaat
     2101 cggcacggca accccggctc atagcgtgaa ccaggcagat tatccggact attactttcg
     2161 tattaccaaa tctgaacaca tgacggaact gaaagaaaaa ttcaaacgta tgtgcgataa
     2221 aagtatgatt aaaaaacgct acatgtacct gaccgaagaa atcctgaaag aaaacccgaa
     2281 tatgtgtgcc tacatggcac cgagcctgga tgcgcgccag gacattgtgg ttgtcgaagt
     2341 tccgaaactg ggtaaagaag cggccaccaa agccatcaaa gaatggggcc aaccgaaatc
     2401 aaaaattacg cacctgatct tttgcaccac gtcgggtgtg gatatgccgg gtgcagacta
     2461 tcagctgacc aaactgctgg gtctgcgtcc gagcgttaaa cgctttatga tgtaccagca
     2521 aggctgcttc gcaggcggta cggtcctgcg tctggctaaa gatctggcgg aaaacaataa
     2581 aggtgctcgc gttctggtgg tttgtagtga aattaccgct gtcacgtttc gtggtccggc
     2641 ggatacccat ctggactccc tggttggcca ggccctgttc ggcgatggtg cagctgcggt
     2701 tatcgtcggc gcagatccgg acacgagtgt ggaacgtccg ctgtatcagc tggtttcaac
     2761 ctcgcaaacg attctgccgg attccgacgg tgcgatcgat ggccatctgc gcgaagtggg
     2821 tctgaccttt cacctgctga aagacgttcc gggcctgatt tcaaaaaaca tcgaaaaaag
     2881 cctgtctgaa gcctttgcac cggttggtat ttcggattgg agctctattt tctggatcgc
     2941 acatccgggc ggtccggcaa tcctggacca ggtggaaagc aaactgggtc tgaaagaaga
     3001 aaaactgaaa gctacccgtc aagtcctgtc tgaatacggc aatatgagtt ccgcgtgtgt
     3061 gctgttcatt ctggatgaaa tgcgcaaaaa atctgccgaa gaagctaaag cgaccacggg
     3121 cgaaggtctg gattggggcg tgctgtttgg tttcggtccg ggtctgaccg tcgaaacggt
     3181 cgtgctgcac agtgtgccga tcaaagcggg cggtggcggt tccggcggtg gtggtagtgg
     3241 tggtggtggc tctccgccgc cggccctgcc gccgaaacgt cgtcgctaac tcgagtctgg
     3301 taaagaaacc gctgctgcga aatttgaacg ccagcacatg gactcgtcta ctagtcgcag
     3361 cttaattaac ctaaactgct gccaccgctg agcaataact agcataaccc cttggggcct
     3421 ctaaacgggt cttgaggggt tttttgctag gatcgagatc gatctcgatc ccgcgaaatt
     3481 aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt
     3541 gtttaacttt aagaaggaga tatacatatg gctgcatcaa tcaccgcaat cactgtggag
     3601 aaccttgaat acccagcggt ggttacctct ccggtcaccg gcaaatcata tttcctcggt
     3661 ggcgctgggg agagaggatt gaccattgaa ggaaacttca tcaagttcac tgccataggt
     3721 gtttatttgg aagatatagc agtggcttca ctagctgcca aatggaaggg taaatcatct
     3781 gaagagttac ttgagaccct tgacttttac agagacatca tctcaggtcc ctttgaaaag
     3841 ttaattagag ggtcaaagat tagggaattg agtggtcctg agtactcaag gaaggttatg
     3901 gagaactgtg tggcacactt gaaatcagtt ggaacttatg gagatgcaga agctgaagct
     3961 atgcaaaaat ttgctgaagc tttcaagcct gttaattttc cacctggtgc ctctgttttc
     4021 tacaggcaat cacctaatgg aatattaggg cttagtttct ctccggatac aagtatacca
     4081 gaaaaggagg ctgcactcat agagaacaag gcagtttcat cagcagtgtt ggagactatg
     4141 atcggcgagc acgctgtttc ccctgatctt aagcgctgtt tagctgcaag attacctgcg
     4201 ttgttgaacg agggtgcttt caagattgga aactgaacta gtcgcagctt aattaaccta
     4261 aactgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
     4321 gaggggtttt ttgctagcga aaggaggagt cgactatatc cggattggcg aatgggacgc
     4381 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     4441 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     4501 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     4561 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     4621 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     4681 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     4741 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     4801 gaattttaac aaaatattaa cgtttacaat ttctggcggc acgatggcat gagattatca
     4861 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     4921 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     4981 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     5041 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     5101 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     5161 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     5221 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     5281 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     5341 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     5401 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     5461 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     5521 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     5581 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     5641 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     5701 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     5761 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     5821 caatcatgat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     5881 tatttagaaa aataaacaaa taggtcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5941 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6001 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6061 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6121 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6181 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6241 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6301 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6361 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6421 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6481 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6541 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6601 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     6661 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     6721 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     6781 atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc
     6841 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
     6901 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     6961 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     7021 ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag
     7081 atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg
     7141 cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc
     7201 gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc
     7261 acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa
     7321 ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc
     7381 gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg
     7441 aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg
     7501 aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt
     7561 cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg
     7621 gtcctcaacg acaggagcac gatcatgcta gtcatgcccc gcgcccaccg gaaggagctg
     7681 actgggttga aggctctcaa gggcatcggt cgagatcccg gtgcctaatg agtgagctaa
     7741 cttacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     7801 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt
     7861 ggtttttctt ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg
     7921 agagagttgc agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat
     7981 ggtggttaac ggcgggatat aacatgagct gtcttcggta tcgtcgtatc ccactaccga
     8041 gatgtccgca ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc ccagcgccat
     8101 ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc tcattcagca tttgcatggt
     8161 ttgttgaaaa ccggacatgg cactccagtc gccttcccgt tccgctatcg gctgaatttg
     8221 attgcgagtg agatatttat gccagccagc cagacgcaga cgcgccgaga cagaacttaa
     8281 tgggcccgct aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag
     8341 tcgcgtaccg tcttcatggg agaaaataat actgttgatg ggtgtctggt cagagacatc
     8401 aagaaataac gccggaacat tagtgcaggc agcttccaca gcaatggcat cctggtcatc
     8461 cagcggatag ttaatgatca gcccactgac gcgttgcgcg agaagattgt gcaccgccgc
     8521 tttacaggct tcgacgccgc ttcgttctac catcgacacc accacgctgg cacccagttg
     8581 atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga
     8641 ggtggcaacg ccaatcagca acgactgttt gcccgccagt tgttgtgcca cgcggttggg
     8701 aatgtaattc agctccgcca tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg
     8761 gctggcctgg ttcaccacgc gggaaacggt ctgataagag acaccggcat actctgcgac
     8821 atcgtataac gttactggtt tcacattcac caccctgaat tgactctctt ccgggcgcta
     8881 tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg tccgggatct cgacgctctc
     8941 ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg
     9001 ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc
     9061 ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt
     9121 ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc
     9181 cggccacgat gcgtccggcg tagcctagga tcgagatcga tctcgatccc gcgaaattaa
     9241 tacgactcac tata
//
