LOCUS       sequence-142129-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FAM83A
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             88..108
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             726..743
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(796..814)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 cctctagaat aattttgttt actttaagaa ggagatatac atatgcacca tcatcatcat
       61 cattcttctg gtgtagatct gggtaccgag aacctgtact tccaatccat ggcctcagct
      121 gagaagccct acctgaagga aaaatccagc gccactgtgt acttccagac cgtcaagcac
      181 aacaacatca gagacctcgt ccgccgctgc atcacccgga ctagccaggt cctggtcatc
      241 ctgatggatg tgttcacgga tgtggagatc ttctgtgaca ttctagaggc agccaacaag
      301 cgtggggtgt tcgtttgtgt gctcctggac cagggaggtg tgaagctctt ccaggagatg
      361 tgtgacaaag tccagatctc tgacagtcac ctcaagaaca tttccatccg gagtgtggaa
      421 ggagagatat actgtgccaa gtcaggcagg aaattcgctg gccaaatccg ggagaagttc
      481 atcatctcgg actggagatt tgtcctgtct ggatcttaca gcttcacctg gctctgcgga
      541 cacgtgcacc ggaacatcct ctccaagttc acaggccagg cggtggagct gtttgacgag
      601 gagttccgcc acctctacgc ctcctccaag cctgtgatgg gcctgaagtc cccgcggctg
      661 tgacagtaaa ggtggatacg gatccgaatt cgagctccgt cgacaagctt gcggccgcac
      721 tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg
      781 agttggctgc tgccaccgct gagcaataac tagcataac
//
