LOCUS       sequence-142134-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gRNA_A to knockout human AMPK alpha 1 using Cas9n.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Toyama EQ, Herzig S, Courchet J, Lewis TL Jr, Loson OC, Hellberg K, 
            Young NP, Chen H, Polleux F, Chan DC, Shaw RJ
  TITLE     Metabolism. AMP-activated protein kinase mediates mitochondrial 
            fission in response to energy stress.
  JOURNAL   Science. 2016 Jan 15;351(6270):275-81. doi: 10.1126/science.aab4138.
  PUBMED    26816379
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-01-15- 15"; volume: "351"; 
            issue: "6270"; pages: "275-81"
COMMENT     Sequence Label: pX462-hPRKAA1-gRNA_A
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     129..148
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        227..302
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        399..684
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 atcaggctgt tagagagata attggaatta atttgactgt aaacacaaag atattagtac
       61 aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt
      121 ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat
      181 atatcttgtg gaaaggacga aacaccggct gtcgccatct ttctccgttt tagagctaga
      241 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
      301 gcttttttgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg tttttagcgc
      361 gtgcgccaat tctgcagaca aatggctcta gaggtacccg ttacataact tacggtaaat
      421 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaatagt aacgccaata
      481 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      541 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      601 gcctggcatt gtgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      661 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      721 atctcccccc cctccccacc cccaattttg
//
