LOCUS       sequence-142135-         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gRNA_A to knockout human AMPK alpha 2 using Cas9n..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Toyama EQ, Herzig S, Courchet J, Lewis TL Jr, Loson OC, Hellberg K, 
            Young NP, Chen H, Polleux F, Chan DC, Shaw RJ
  TITLE     Metabolism. AMP-activated protein kinase mediates mitochondrial 
            fission in response to energy stress.
  JOURNAL   Science. 2016 Jan 15;351(6270):275-81. doi: 10.1126/science.aab4138.
  PUBMED    26816379
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-01-15- 15"; volume: "351"; 
            issue: "6270"; pages: "275-81"
COMMENT     Sequence Label: pX462-hPRKAA2-gRNA_A
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     100..119
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        199..274
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        371..656
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt
       61 cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta
      121 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccgtc
      181 agccatcttc ggcgcgcggt tttagagcta gaaatagcaa gttaaaataa ggctagtccg
      241 ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt gttttagagc tagaaatagc
      301 aagttaaaat aaggctagtc cgtttttagc gcgtgcgcca attctgcaga caaatggctc
      361 tagaggtacc cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      421 cccgcccatt gacgtcaata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      481 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      541 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttgtgcccag tacatgacct
      601 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg
      661 aggtgagccc cacgttctgc ttcactctcc ccatctcccc ccccctccc
//
