LOCUS       sequence-142136-         719 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Intermediate cloning vector, with MCS2 containing inducible unstable
            peptide driven by pSTL1 (UbiY 2xNLSs SZ1)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Aymoz D, Wosika V, Durandau E, Pelet S
  TITLE     Real-time quantification of protein expression at the single-cell 
            level via dynamic protein synthesis translocation reporters.
  JOURNAL   Nat Commun. 2016 Apr 21;7:11304. doi: 10.1038/ncomms11304.
  PUBMED    27098003
REFERENCE   2  (bases 1 to 719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-04-21"; volume: "7:11304. doi"; pages: " 
            10.1038/ncomms11304"
COMMENT     Sequence Label: pDA125
FEATURES             Location/Qualifiers
     source          1..719
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             348..368
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             384..404
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(673..691)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 agactttgac cggtaaaacc ataacattgg aagttgaatc ttccgatacc atcgacaacg
       61 ttaagtcgaa aattcaagac aaggaaggta tccctccaga tcaacaaaga ttgatctttg
      121 ccggtaagca gctagaagac ggtagaacgc tgtctgatta caacattcag aaggagtcca
      181 ccttacatct tgtgctaagg ctaagaggtg gttaccataa atcaggtgcg tggctgcttc
      241 cagttagttt ggtaaagaga aaaacgaccc tagcacctaa tactcaaaca gcttccccca
      301 gggctttggc cgattcttta atgcagggaa agcttatgag atccgaaccc aagaagaagc
      361 ggaaggtggg ggctggtgct gaacccaaga agaagcggaa ggtgggggga tctagtgtcg
      421 acggtggcag caatcaaact tccttatata aaaaagctgg aagtgccgca gcacctttta
      481 caatggaatt taaaagatct aacctggttg cgcagctcga aaacgaagtt gcgtctctgg
      541 aaaatgagaa cgaaaccctg aagaaaaaga acctgcacaa aaaagacctg atcgcgtacc
      601 tggagaaaga aatcgcgaat ctgcgtaaga aaatcgaaga ataagctagc ctcgagtcat
      661 gtaattagtt atgtcacgct tacattcacg ccctcccccc acatccgctc taaccgaaa
//
