LOCUS       sequence-142140-         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Intermediate cloning vector, with MCS2 containing inducible unstable
            peptide driven by pGPD1 (UbiY 2xNLSs SZ3)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Aymoz D, Wosika V, Durandau E, Pelet S
  TITLE     Real-time quantification of protein expression at the single-cell 
            level via dynamic protein synthesis translocation reporters.
  JOURNAL   Nat Commun. 2016 Apr 21;7:11304. doi: 10.1038/ncomms11304.
  PUBMED    27098003
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-04-21"; volume: "7:11304. doi"; pages: " 
            10.1038/ncomms11304"
COMMENT     Sequence Label: pDA186
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             332..352
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             368..388
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(645..663)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 aaccataaca ttggaagttg aatcttccga taccatcgac aacgttaagt cgaaaattca
       61 agacaaggaa ggtatccctc cagatcaaca aagattgatc tttgccggta agcagctaga
      121 agacggtaga acgctgtctg attacaacat tcagaaggag tccaccttac atcttgtgct
      181 aaggctaaga ggtggttacc ataaatcagg tgcgtggctg cttccagtta gtttggtaaa
      241 gagaaaaacg accctagcac ctaatactca aacagcttcc cccagggctt tggccgattc
      301 tttaatgcag ggaaagctta tgagatccga acccaagaag aagcggaagg tgggggctgg
      361 tgctgaaccc aagaagaagc ggaaggtggg gggatctagt gtcgacggtg gcagcaatca
      421 aacttcctta tataaaaaag ctggaagtgc cgcagcacct tttacaatgg aatttaaaag
      481 atctaacgaa gttaccactc tggagaatga cgctgcgttc atcgaaaatg aaaacgctta
      541 cctggaaaaa gaaatcgcgc gtctgcgtaa agaaaaagcg gcgctgcgca accgtctggc
      601 gcacaaaaaa tgataagcta gcctcgagtc atgtaattag ttatgtcacg cttacattca
      661 cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag
      721 gtccctattt attt
//
