LOCUS       sequence-142142-         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Intermediate cloning vector, with MCS2 containing inducible stable 
            peptide driven by pSTL1 (Venus 2xNLSs SZ1)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Aymoz D, Wosika V, Durandau E, Pelet S
  TITLE     Real-time quantification of protein expression at the single-cell 
            level via dynamic protein synthesis translocation reporters.
  JOURNAL   Nat Commun. 2016 Apr 21;7:11304. doi: 10.1038/ncomms11304.
  PUBMED    27098003
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-04-21"; volume: "7:11304. doi"; pages: " 
            10.1038/ncomms11304"
COMMENT     Sequence Label: pDA171
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..21
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             37..57
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cccaagaaga agcggaaggt gggggctggt gctgaaccca agaagaagcg gaaggtgggg
       61 ggatctagta gatctgcttc taaaggtgaa gaattattca ctggtgttgt cccaattttg
      121 gttgaattag atggtgatgt taatggtcac aaattttctg tctccggtga aggtgaaggt
      181 gatgctactt acggtaaatt gaccttaaaa ttgatttgta ctactggtaa attgccagtt
      241 ccatggccaa ccttagtcac tactttaggt tatggtttgc aatgttttgc tagataccca
      301 gatcatatga aacaacatga ctttttcaag tctgccatgc cagaaggtta tgttcaagaa
      361 agaactattt ttttcaaaga tgacggtaac tacaagacca gagctgaagt caagtttgaa
      421 ggtgatacct tagttaatag aatcgaatta aaaggtattg attttaaaga agatggtaac
      481 attttaggtc acaaattgga atacaactat aactctcaca atgtttacat cactgctgac
      541 aaacaaaaga atggtatcaa agctaacttc aaaattagac acaacattga agatggtggt
      601 gttcaattag ctgaccatta tcaacaaaat actccaattg gtgatggtcc agtcttgtta
      661 ccagacaacc attacttatc ctatcaatct gccttatcca aagatccaa
//
