LOCUS       sequence-142151-         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression vector containing flag tagged S155,172A MFF 
            isoform 5 mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Toyama EQ, Herzig S, Courchet J, Lewis TL Jr, Loson OC, Hellberg K, 
            Young NP, Chen H, Polleux F, Chan DC, Shaw RJ
  TITLE     Metabolism. AMP-activated protein kinase mediates mitochondrial 
            fission in response to energy stress.
  JOURNAL   Science. 2016 Jan 15;351(6270):275-81. doi: 10.1126/science.aab4138.
  PUBMED    26816379
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-01-15- 15"; volume: "351"; 
            issue: "6270"; pages: "275-81"
COMMENT     Sequence Label: pLenti.PGK.blast-Flag-MFF-S155,172A
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    81..105
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             114..137
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 accgaatcac cgacctctct ccccaggggg atccaccggt tcgtcgacta gtccagtgtg
       61 gtggaattct gcagatatca acaagtttgt acaaaaaagc aggcttcacc atggattaca
      121 aggacgacga tgacaagggc ggatctatgg cagaaattag tcgaattcag tacgaaatgg
      181 aatatactga aggcattagt cagcgaatga gggtcccaga aaagttaaaa gtagcaccgc
      241 caaacgctga cctggaacaa ggattccaag aaggagttcc aaatgctagt gtgataatgc
      301 aagttccgga gaggattgtt gtagcaggaa ataatgaaga tgtttcattt tcaagaccag
      361 cagatcttga ccttattcag tcaactccct ttaaacccct ggcactgaaa acaccacctc
      421 gtgtacttac gctgagtgaa agaccactag attttctgga tttagaaaga cctcctacaa
      481 cccctcaaaa tgaagaaatc cgagcagttg gcagactaaa aagagagcgg gctatgagtg
      541 aaaatgctgt tcgccaaaat ggacagctgg tcagaaatga tgctctacct gtgttgcgtg
      601 gtgggtctgc tgccgccact tctaatcctc atcatgacaa cgtcaggtat ggcatttcaa
      661 atatagatac aaccattgaa ggaacgtcag atgacctgac tgttgtagat gcagcttcac
      721 taagacgaca gataatcaaa ctaaatagac gtctac
//
