LOCUS       sequence-142152-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression vector encoding untagged WT human AMPK alpha 
            1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Toyama EQ, Herzig S, Courchet J, Lewis TL Jr, Loson OC, Hellberg K, 
            Young NP, Chen H, Polleux F, Chan DC, Shaw RJ
  TITLE     Metabolism. AMP-activated protein kinase mediates mitochondrial 
            fission in response to energy stress.
  JOURNAL   Science. 2016 Jan 15;351(6270):275-81. doi: 10.1126/science.aab4138.
  PUBMED    26816379
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-01-15- 15"; volume: "351"; 
            issue: "6270"; pages: "275-81"
COMMENT     Sequence Label: pLenti.CMV/TO_PRKAA1
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    55..79
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aattttgtaa tccagaggtt gattgtcgag cggccgccac tgtgctggat atcaaccact
       61 ttgtacaaga aagctgggtt ctattgtgca agaattttaa ttagatttgc acacatctca
      121 aaaaattcta ttgtgtgact tccaggtctt ggagttaggt caacaggaga agagtcaagt
      181 gaggtcacag atgaggtaag agaaacttct gaggattttc cttgagcctc agcatctgaa
      241 tcactccttt ggcaagatcg atagttgcta actgatcccg atctctgtgg agtagcagtc
      301 cctgatttgg cttctgtaat ttcatcatca atactacgga aatccagtag ataagttcta
      361 ctatccactt ggtataactg tagactcatt ttggagtaag tgcttgtcac aggattcttc
      421 cttcgtacac gcaaataata tgggtttaca accttccatt cataatccaa ttgtttgatt
      481 gctctacata cttctgccat aatatcattt ggtcgacttt gacttctaat tcctaaatgc
      541 cattttgctt tccttacacc ttggtgtttg gatttctgtg gatttaattc atcaagggta
      601 tggcgtgccc ttggtgtttc agcaaccaag aatggtactc tttcaggatg gggccgagtc
      661 aggtgatgat catcaagaaa agaatcaggt gggcttgtcg ccaaatagaa atctttggct
      721 tcattcatta ttctcctgtt atctattatg agatggtagg caactgccaa aggatcctgg
      781 tgatttctgt tgtaaagaca gctgagaact tcctcttctg agcactcaaa cttttcacat
      841 acttctttta aggcttcatc atcaatcatg gttgaactat atgatggatc ctcaggaaag
      901 agatattttg gaaggtcctg
//
