LOCUS       sequence-142157-         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression vector containing flag tagged S155,172D MFF 
            isoform 5 mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Toyama EQ, Herzig S, Courchet J, Lewis TL Jr, Loson OC, Hellberg K, 
            Young NP, Chen H, Polleux F, Chan DC, Shaw RJ
  TITLE     Metabolism. AMP-activated protein kinase mediates mitochondrial 
            fission in response to energy stress.
  JOURNAL   Science. 2016 Jan 15;351(6270):275-81. doi: 10.1126/science.aab4138.
  PUBMED    26816379
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-01-15- 15"; volume: "351"; 
            issue: "6270"; pages: "275-81"
COMMENT     Sequence Label: pLenti.PGK.blast-Flag-MFF-S155,172D
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    89..113
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             122..145
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cctcgttgac cgaatcaccg acctctctcc ccagggggat ccaccggttc gtcgactagt
       61 ccagtgtggt ggaattctgc agatatcaac aagtttgtac aaaaaagcag gcttcaccat
      121 ggattacaag gacgacgatg acaagggcgg atctatggca gaaattagtc gaattcagta
      181 cgaaatggaa tatactgaag gcattagtca gcgaatgagg gtcccagaaa agttaaaagt
      241 agcaccgcca aacgctgacc tggaacaagg attccaagaa ggagttccaa atgctagtgt
      301 gataatgcaa gttccggaga ggattgttgt agcaggaaat aatgaagatg tttcattttc
      361 aagaccagca gatcttgacc ttattcagtc aactcccttt aaacccctgg cactgaaaac
      421 accacctcgt gtacttacgc tgagtgaaag accactagat tttctggatt tagaaagacc
      481 tcctacaacc cctcaaaatg aagaaatccg agcagttggc agactaaaaa gagagcggga
      541 tatgagtgaa aatgctgttc gccaaaatgg acagctggtc agaaatgatg atctacctgt
      601 gttgcgtggt gggtctgctg ccgccacttc taatcctcat catgacaacg tcaggtatgg
      661 catttcaaat atagatacaa ccattgaagg aacgtcagat gacctgactg ttgtagatgc
      721 agcttcacta agacgacaga taatcaaact aaatagacgt ctac
//
