LOCUS       sequence-142173-         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP 
            from EFS promoter. 3rd generation lentiviral backbone. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Chen S, Sanjana NE, Zheng K, Shalem O, Lee K, Shi X, Scott DA, Song 
            J, Pan JQ, Weissleder R, Lee H, Zhang F, Sharp PA
  TITLE     Genome-wide CRISPR screen in a mouse model of tumor growth and 
            metastasis.
  JOURNAL   Cell. 2015 Mar 12;160(6):1246-60. doi: 10.1016/j.cell.2015.02.038. 
            Epub 2015 Mar 5.
  PUBMED    25748654
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.02.038"; journalName: "Cell"; date: 
            "2015-03-12- 12"; volume: "160"; issue: "6"; pages: "1246-60"
COMMENT     Sequence Label: lentiCas9-EGFP
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     219..240
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(283..339)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(349..372)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(373..420)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg
       61 gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac
      121 gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc
      181 ctcgccctcg ccagacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag
      241 gatgggcacc accccggtga acagctcctc gcccttgctc accggtccag gattctcttc
      301 gacatctccg gcttgtttca gcagagagaa gtttgttgcg ccggatccct tatcgtcatc
      361 gtctttgtaa tctttcttct tcttagcctg tccagccttc tttgtggcgg caggtcgctt
      421 gtcgcctccc agctgagaca ggtcgatccg tgtctcgtac aggccggtga tgctctggtg
      481 gatcagggtg gcgtccagca cctctttggt gctggtgtac ctcttccggt cgatggtggt
      541 gtcaaagtac ttgaaggcgg caggggctcc cagattggtc agggtaaaca ggtggatgat
      601 attctcggcc tgctctctga tgggcttatc ccggtgcttg ttgtaggcgg acagcacttt
      661 gtccagatta gcgtcggcca ggatcactct cttggagaac tcgctgatct gctcgatgat
      721 ctcgtccagg tagtgcttgt gctgttccac aaacagctgt ttctgctcat tatcctcggg
      781 ggagcccttc agcttctcat agtggctggc caggtacagg aagttcacat atttggaggg
      841 cagggccagt tcgtttccct tctgcagttc gccggcagag gccagcattc tcttccggcc
      901 gttt
//
