LOCUS       sequence-142175-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression vector containing flag tagged S155,172E MFF 
            isoform 5 mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Toyama EQ, Herzig S, Courchet J, Lewis TL Jr, Loson OC, Hellberg K, 
            Young NP, Chen H, Polleux F, Chan DC, Shaw RJ
  TITLE     Metabolism. AMP-activated protein kinase mediates mitochondrial 
            fission in response to energy stress.
  JOURNAL   Science. 2016 Jan 15;351(6270):275-81. doi: 10.1126/science.aab4138.
  PUBMED    26816379
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-01-15- 15"; volume: "351"; 
            issue: "6270"; pages: "275-81"
COMMENT     Sequence Label: pLenti.PGK.blast-Flag-MFF-S155,172E
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    84..108
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             117..140
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttgaccgaat caccgacctc tctccccagg gggatccacc ggttcgtcga ctagtccagt
       61 gtggtggaat tctgcagata tcaacaagtt tgtacaaaaa agcaggcttc accatggatt
      121 acaaggacga cgatgacaag ggcggatcta tggcagaaat tagtcgaatt cagtacgaaa
      181 tggaatatac tgaaggcatt agtcagcgaa tgagggtccc agaaaagtta aaagtagcac
      241 cgccaaacgc tgacctggaa caaggattcc aagaaggagt tccaaatgct agtgtgataa
      301 tgcaagttcc ggagaggatt gttgtagcag gaaataatga agatgtttca ttttcaagac
      361 cagcagatct tgaccttatt cagtcaactc cctttaaacc cctggcactg aaaacaccac
      421 ctcgtgtact tacgctgagt gaaagaccac tagattttct ggatttagaa agacctccta
      481 caacccctca aaatgaagaa atccgagcag ttggcagact aaaaagagag cgggagatga
      541 gtgaaaatgc tgttcgccaa aatggacagc tggtcagaaa tgatgaacta cctgtgttgc
      601 gtggtgggtc tgctgccgcc acttctaatc ctcatcatga caacgtcagg tatggcattt
      661 caaatataga tacaaccatt gaaggaacgt cagatgacct gactgttgta gatgcagctt
      721 cactaagacg acagataatc aaactaaata gacgtctaca acttctggaa gaggagaaca
      781 aagaacgtgc taaaagagaa atggtcatgt attcaattac tgtagctttc tggctgctta
      841 atagc
//
