LOCUS       sequence-142180-        1064 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for SICLOPPS-mediated protein circularization using the 
            Nostoc punctiforme DnaE intein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Waldhauer MC, Schmitz SN, Ahlmann-Eltze C, Gleixner JG, Schmelas CC,
            Huhn AG, Bunne C, Buscher M, Horn M, Klughammer N, Kreft J, Schafer 
            E, Bayer PA, Kramer SG, Neugebauer J, Wehler P, Mayer MP, Eils R, Di
            Ventura B
  TITLE     Backbone circularization of Bacillus subtilis family 11 xylanase 
            increases its thermostability and its resistance against 
            aggregation.
  JOURNAL   Mol Biosyst. 2015 Nov 10;11(12):3231-43. doi: 10.1039/c5mb00341e.
  PUBMED    26434634
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2015-11-10- 10"; volume: "11"; issue: "12"; 
            pages: "3231-43"
COMMENT     Sequence Label: pCIRCNpu
FEATURES             Location/Qualifiers
     source          1..1064
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..32
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(36..79)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     87..106
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        87..105
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    106..130
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    133..154
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     RBS             161..183
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    405..426
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(441..446,447..464,465..471)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    479..495
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             513..524
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 attaacctat aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc
       61 tttcgctaag gatgatttct ggaatttaat acgactcact ataggggaat tgtgagcgga
      121 taacaattcc ccgaattcgc ggccgcttct agagaataat tttgtttaac tttaagaagg
      181 agatactaga tgatcaaaat agccacacgt aaatatttag gcaaacaaaa tgtctatgac
      241 attggagttg agcgcgacca taattttgca ctcaaaaatg gcttcatagc ttccaacaga
      301 gacccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
      361 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
      421 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
      481 gtgagcggat aacaatttca cacatactag agaaagagga gaaatactag atggcttcct
      541 ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg
      601 gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg
      661 ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc
      721 agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg gactacctga
      781 aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg
      841 ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac aaagttaaac
      901 tgcgtggtac caacttcccg tccgatggtc cggttatgca gaaaaaaacc atgggttggg
      961 aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa atcaaaatgc
     1021 gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaa
//
