LOCUS       sequence-142181-         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for SICLOPPS-mediated protein circularization using the 
            Nostoc punctiforme DnaE intein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Waldhauer MC, Schmitz SN, Ahlmann-Eltze C, Gleixner JG, Schmelas CC,
            Huhn AG, Bunne C, Buscher M, Horn M, Klughammer N, Kreft J, Schafer 
            E, Bayer PA, Kramer SG, Neugebauer J, Wehler P, Mayer MP, Eils R, Di
            Ventura B
  TITLE     Backbone circularization of Bacillus subtilis family 11 xylanase 
            increases its thermostability and its resistance against 
            aggregation.
  JOURNAL   Mol Biosyst. 2015 Nov 10;11(12):3231-43. doi: 10.1039/c5mb00341e.
  PUBMED    26434634
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2015-11-10- 10"; volume: "11"; issue: "12"; 
            pages: "3231-43"
COMMENT     Sequence Label: pCIRCNpu
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..22
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(46..103)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    104..124
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      complement(474..501)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(517..588)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 acgcagcgag tcagtgagcg aggaagcctg cataacgcga agtaatcttt tcggttttaa
       61 agaaaaaggg cagggtggtg acaccttgcc cttttttgcc ggactgcagc ggccgctact
      121 agtactatca gtcgtgcatt tcaccgattt attaattcgg caaattatca acccgcatca
      181 aatccaattc acgttcaaat atttcatcaa ttggcaacat ttgaccatca acagtcataa
      241 acttatggtc ttttgttgcc cgaatcaatg aaccatcttc caaacaatac tcaaacacct
      301 cttgttctcc gcgatcgtgc cattgtgcta caggttgtgt ataaatattt ccattattat
      361 caacgctata aacagtacat tcgatgcgct tttctacaat tttaccaatc ggtaataatc
      421 catattctac tgtcaatatt tccgtttcat agcttaagca tgagacctat aaacgcagaa
      481 aggcccaccc gaaggtgagc cagtgtgact ctagtagaga gcgttcaccg acaaacaaca
      541 gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctggctct
      601 agtagcgatc tacactagca ctatcagcgt tattaagcac cggtggagtg acgaccttca
      661 gcacgttcgt actgttcaac gatggtgtag tcttcgttgt gggaggtgat gtccagtttg
      721 atgtcggttt tgtaagcacc cggcagctga accggttttt tagccatgta ggtggtttta
      781 acttcagcgt cgtagtgacc accgtctttc agtttcagac gcattttgat ttcacctttc
      841 agagcaccgt cttccgggta catacgttcg gtggaagctt ccca
//
