LOCUS       sequence-142189-         621 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression vector containing Flag tagged S155A MFF 
            isoform 5 mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Toyama EQ, Herzig S, Courchet J, Lewis TL Jr, Loson OC, Hellberg K, 
            Young NP, Chen H, Polleux F, Chan DC, Shaw RJ
  TITLE     Metabolism. AMP-activated protein kinase mediates mitochondrial 
            fission in response to energy stress.
  JOURNAL   Science. 2016 Jan 15;351(6270):275-81. doi: 10.1126/science.aab4138.
  PUBMED    26816379
REFERENCE   2  (bases 1 to 621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-01-15- 15"; volume: "351"; 
            issue: "6270"; pages: "275-81"
COMMENT     Sequence Label: pQCXIB Flag MFF S155A
FEATURES             Location/Qualifiers
     source          1..621
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        258..461
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     412..432
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    463..481
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    484..502
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    543..567
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             576..599
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
       61 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      121 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      181 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      241 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      301 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      361 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      421 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctccctatc agtgatagag
      481 atctccctat cagtgataga gatcgtcgac tagtccagtg tggtggaatt ctgcagatat
      541 caacaagttt gtacaaaaaa gcaggcttca ccatggatta caaggacgac gatgacaagg
      601 gcggatctat ggcagaaatt a
//
