LOCUS       sequence-142193-        1072 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing iBlueberry and mCherry in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Yu D, Dong Z, Gustafson WC, Ruiz-Gonzalez R, Signor L, Marzocca F, 
            Borel F, Klassen MP, Makhijani K, Royant A, Jan YN, Weiss WA, Guo S,
            Shu X
  TITLE     Rational design of a monomeric and photostable far-red fluorescent 
            protein for fluorescence imaging in vivo.
  JOURNAL   Protein Sci. 2016 Feb;25(2):308-15. doi: 10.1002/pro.2843. Epub 2015
            Dec 31.
  PUBMED    26549191
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2843"; 
            journalName: "Protein Sci"; date: "2016-02"; volume: "25"; issue: 
            "2"; pages: "308-15"
COMMENT     Sequence Label: pcDNA3.1_LifeAct-iBlueberry
FEATURES             Location/Qualifiers
     source          1..1072
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             191..244
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             251..961
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(396..414)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     664..683
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(993..1010)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gggcgttggc gcgaccctgg tttgctctct gatggtgtct ggtcgtctgt ggggtctgat
       61 cgcttgccac cactacgaac cgcgcttcgt tccgttccac attcgcgctg ctggcgaagc
      121 gctggcggaa acttgtgcga tccgcatcgc gacgctggag agctttgcac agtctcagtc
      181 caaaggatcc gagggcagag gaagtctgct aacatgcggt gacgtcgagg agaatcctgg
      241 cccagaattc atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
      301 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
      361 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
      421 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
      481 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
      541 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
      601 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
      661 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
      721 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
      781 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
      841 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
      901 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta
      961 atctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     1021 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ct
//
