LOCUS       sequence-142197-         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains sgRNA which targets GFPmut3b. Under an aTc inducible 
            promoter. Suicide vector inserts into slr0230 site of Synechocystis.
            Carries kanamycin resistance. Propogates in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Yao L, Cengic I, Anfelt J, Hudson EP
  TITLE     Multiple Gene Repression in Cyanobacteria Using CRISPRi.
  JOURNAL   ACS Synth Biol. 2015 Dec 28.
  PUBMED    26689101
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Dec 28."
COMMENT     Sequence Label: pMD19T-slr0230-PL22-sgRNANT1-KmR
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tctggcacga caggtttccc gactggggga acaagctgaa tctgggcatc ccggtaattg
       61 gaatccgact ccaagccgat taaagctcct tccgtatcca ggggaattaa acattggggg
      121 ctgtcactgc cggcccgcca caatagggga gggttatggg cggcgttgct ataggaaagt
      181 atgctcgttt cggggttata ttcagaataa aacaaggtga caaagcgatg ggaattttcc
      241 aggtcggcat acattacccg gttgaggtgg ttaagaattt gggctggact atggcggttc
      301 agcacttctg cccgcagcat ccccctagtc atggtcataa ttagacccgc cggtactcct
      361 ttacccatga catcgccgat gacaatactc cagggcaccc ccaaatggct tgtgttacga
      421 cataaccaat ccccctggcg cagttggtca tagttggcag gaatgaaatc gtagtaatcc
      481 ccacctaccc gactggcggt tttacactgg gcagcaatgt ccaaaccatt aatttgggga
      541 caacaacggg gcaatagttg attttgaatt tctgaagcaa tttccaattc ccgatcctga
      601 ctttctctgg atctcaactt ttggtttaaa tcactattgg cgatcgccac agcggtttga
      661 tcagaaatta gctgtagtaa cttacgacgg gtgggagtcc agctatagtt acgatcttga
      721 ctaaagatat ataagcggcc cacttcctct tggtgactca aaatgggggt gttataacag
      781 gcgatcgccg gtaattcttg gcgcaattga cggtcaaaat aactcaggac agtggaagaa
      841 tccaatgtga ggttaacctg gcgggccttc tgcaaagcgc ctttaatggt gcccttaaga
      901 ctatttttgt ggccatggat ttgttcaaag atagatattt ccccctctct catta
//
