LOCUS       sequence-142207-         602 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses WT DDX3X in mammalian cells under blasticidin selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Wang T, Birsoy K, Hughes NW, Krupczak KM, Post Y, Wei JJ, Lander ES,
            Sabatini DM
  TITLE     Identification and characterization of essential genes in the human 
            genome.
  JOURNAL   Science. 2015 Oct 15. pii: aac7041.
  PUBMED    26472758
REFERENCE   2  (bases 1 to 602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2015 Oct 15. pii: aac7041."
COMMENT     Sequence Label: pMXs-DDX3X
FEATURES             Location/Qualifiers
     source          1..602
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             520..585
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 gcagcaagag gactggacat ttcaaatgtg aaacatgtta tcaattttga cttgccaagt
       61 gatattgaag aatatgtaca tcgtattggt cgtacgggac gtgtaggaaa ccttggcctg
      121 gcaacctcat tctttaacga gaggaacata aatattacta aggatttgtt ggatcttctt
      181 gttgaagcta aacaagaagt gccgtcttgg ttagaaaaca tggcttatga acaccactac
      241 aagggtagca gtcgtggacg ttctaagagt agcagattta gtggagggtt tggtgccaga
      301 gactaccgac aaagtagcgg tgccagcagt tccagcttca gcagcagccg cgcaagcagc
      361 agccgcagtg gcggaggtgg ccacggtagc agcagaggat ttggtggagg tggctatgga
      421 ggcttttaca acagtgatgg atatggagga aattataact cccagggggt tgactggtgg
      481 ggtaacgcgg ccgcaggagg cggtggaggc ggtgcaggcg actacaaaga ccatgacggt
      541 gattataaag atcatgacat tgattacaag gatgacgatg acaagtaata ataaattccg
      601 cc
//
