LOCUS       sequence-142208-       12442 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express the inhibitory scaffold gephyrin tagged to teal 
            fluorescent protein. Expression is Cre dependant. Plasmid can also 
            be used to make lentivirus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12442)
  AUTHORS   Villa KL, Berry KP, Subramanian J, Cha JW, Oh WC, Kwon HB, Kubota Y,
            So PT, Nedivi E
  TITLE     Inhibitory Synapses Are Repeatedly Assembled and Removed at 
            Persistent Sites In Vivo.
  JOURNAL   Neuron. 2016 Feb 3. pii: S0896-6273(16)00011-8. doi: 
            10.1016/j.neuron.2016.01.010.
  PUBMED    26853302
REFERENCE   2  (bases 1 to 12442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Feb 3. pii: S0896-6273(16)00011-8. doi: 
            10.1016/j.neuron.2016.01.010."
COMMENT     Sequence Label: FU- dio Teal-gephyrin - W
FEATURES             Location/Qualifiers
     source          1..12442
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2611..3821
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3658..3677
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(3874..3907)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(3958..3991)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(6271..6978)
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     regulatory      6975..6984
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    6992..7025
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    7076..7109
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7134..7722
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7187..7207)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7605..7616)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7725..7741)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7726..7742)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8247..8427
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8453..8470)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8459..8683
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8729..9157
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8816..8835)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9026..9047
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9166..9186)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9171..9500
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9351..9486
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9413..9432
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9548..9595
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9614..9988
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10118..10239
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10155..10174)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10209..10228
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10288..10304)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10288..10304)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10301..10323)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10312..10328
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10336..10342,10343..10360,10361..10366))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10381..10402
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10519..10536)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10690..11278)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10770..10789)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11449..12240,12241..12309))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12072..12091
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12310..12414)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agggtgcagc ggcctccgcg ccgggttttg gcgcctcccg
     2641 cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc aggagcgttc
     2701 ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag
     2761 aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg
     2821 ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg
     2881 agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac
     2941 agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc
     3001 gtcacttggt gagttgcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct
     3061 cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag
     3121 gttgccctga actgggggtt ggggggagcg cacaaaatgg cggctgttcc cgagtcttga
     3181 atggaagacg cttgtaaggc gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg
     3241 gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg gaaagctctt attcgggtga
     3301 gatgggctgg ggcaccatct ggggaccctg acgtgaagtt tgtcactgac tggagaactc
     3361 gggtttgtcg tctggttgcg ggggcggcag ttatgcggtg ccgttgggca gtgcacccgt
     3421 acctttggga gcgcgcgcct cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg
     3481 cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg
     3541 gttcgggcct agggtaggct ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg
     3601 gataagtgag gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt aagtagctga
     3661 agctccggtt ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa gttttttagg
     3721 caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaat
     3781 tgtccgctaa attctggccg tttttggctt ttttgttaga cgaagcttgg gctgcaggtc
     3841 gactctagag gatcctctag agtcgactcc ggaataactt cgtataggat actttatacg
     3901 aagttatgca gaatggtagc tggattgtag ctgctattag caatatgaaa cctcttaata
     3961 acttcgtata gcatacatta tacgaagtta tggcgcgccc gtacgtcata gccgtccgat
     4021 gaccatgacg tccaccacct cgcctttgtg aagttccaca tactgctctg tctttggagg
     4081 tagcatcagc aatccattgg cactgcgcat gctcatcaga cggctgctca tctgattacc
     4141 tgtactctgg gcccagggca gtggttcttg gtgatgccaa gtcagtatac accggtggta
     4201 ttctgggcga gggtccagct ttacgtcaca tgataaccta gctttgatga tggttggccg
     4261 aggatccaag ataccctgca tttttctcag tgcgggtaca acaaagaggt tgcaggtgac
     4321 cacagctgat acaggattcc ctggtagtgc aaaaataatt tttcttacac catcaatatc
     4381 caaagttgca aatgttgttg gcaagcctgg cttcataaaa accctgccaa aatggatctg
     4441 agcatgaaga tcgatgtcca gcacctgctt gagatagtcc ttttccccca tggatacacc
     4501 tcctgacgtg atgatgacat cagcacggct gataccctca ttcaaggcat tgagtaagtc
     4561 atctgggttg tctcctacaa ttcccaagtt gattgttggg taaccatgtt cctgaattgt
     4621 tgctagaagt gttgatcgat tgctgtcccg aatctttcct ggtaagaggt catcttcagg
     4681 atttagtagc tcattccctg ttgacataac ggcaaccact ggaaacttat taacttcaac
     4741 ttctgtgaca cctacagttg caaggagacc aatttctgaa gggcccatat gggttccttt
     4801 tgccaaaaca cactcccctc ttttaatgtc gtggccgatg ggtctgatgt cttggcctgg
     4861 ccgagcttgc accagaattc gcacttcaag ttcttcagta ccatcatctg attccctgat
     4921 gagttcagta tcttcaactt gcactactgc atcagcaccg caggggattg gagcacctgt
     4981 tgtaacccgc atcacttgtc cgggcattac tgtctgagtt ggctgttcac cagcttggga
     5041 ttccccaatg atgaaacgat ctcctggacc atcagcagct cgaacagcat agccatcttt
     5101 tactgatgct gggaatgggg gtaggttgtc ttttgcatac acatcttgag caaggactcg
     5161 ccccattcca tctcggtaat tgatgatttc tgtaccaagc accggagtca tctccagaac
     5221 tgtaatgaag gctttgtcca tagacgtcag aggaaaagga gacatgcgat gtcttctagc
     5281 caccttggtg atatctacag cactgtgact ggctcttaga atgttctcct tgctgctgca
     5341 cctggactgg acttttggtg ttggacaaga agctgtagag aggcgagatg tagcctgggc
     5401 acggggcgac tctgaaggag tagtgctaag ggaggctgtg tctcgtggga gcacctgaac
     5461 gccacgagaa atgatggagt ctggaatctt tgcagcaaga gctgctgcag taatatgtga
     5521 tgaggaacta tcttctgtcg aagctacacc actgtctttc ttttcttctt cctcctcttc
     5581 acactgaact cctttgtctt ctgtctgctt atgtgggcta gttgtaggag gtggagaaag
     5641 aggaggtggt ggggaaggta aatcttcaag ttcatcatgc acctccttta cttttacaat
     5701 ggcatcacgt aaaaggtcaa tggcatgagg tagagctggc agtatgaact gaaagcattc
     5761 ctgagatcct ttcttgctac caggcaggtt aattatcaga gttttccctc ttattccaca
     5821 cactggtcta gagagcatgc ccagaggtgt aacattaagt gatcccatca gcattgccag
     5881 ggccatccct ggcgcttccc gttctattac ttcttttgtg gcctctggag tgacatctcg
     5941 tggtgcaaac cctgttccgc cagttgttaa tatcaaatta agttccttct catcacacca
     6001 gtctatgagt gtttccttga tttcttctat ttcatctggt actatcttgt atgctgatat
     6061 agtcccaccc aacaaagaag gatcctggac gagatctttt agatttatcc cactgcggtc
     6121 ttctgcaaga ttcctgaagc agctatcact cactgtgagg actccgacac ggatttgatg
     6181 gtcgtggttt gtgaggatca ttccctcggt cgccatgttt cccctcgtgc cgaattcgaa
     6241 gcttgagctc gagatctgag tccggccgga cttgtacagc tcgtccatgc cgtcggtgga
     6301 gttgcgggcc acggcgctct cgtaaacggt caccttgttg tagtccttgt cgtggttcag
     6361 gatctcgatg cggtggtcca caaagtgata gtcgggcagc ttcaccgcct tcttggccct
     6421 gtagatggtc ttgaagtcaa cgcggtggtg gccgccgccc tccagcagca gcttgtgctt
     6481 gacgtcgccc ttcagcacgc cgtcgcgcac gtacatcctc tcggtggagg cgtcccagcc
     6541 ggtggtcttc ttctgcatca cggggccgtt gggggggaag ttctcgccct tgaggtgtat
     6601 ctcgtagatg aaggagtcct cctccatgga gatgtcggac ttcaccttca cgatgccctt
     6661 gtcctcgaag gtcatggtgc gctcccaaga gtagccctcg gggaaggact gcttgaagta
     6721 gttggggatg tcgtcggggt acttggtgaa ggccctgttg ccgtaggcga acgcggtggt
     6781 cagaatgtcg taggagaagg gcaggggggc tccctccttc acctccaggt tgatggtgtt
     6841 ggtgccgtcg tagggcttgc cctcgccctc gccctcgatc acgaaggcgt ggccattcac
     6901 gttgccctcc atcttcagct tgatcttcat gtcgggcttg attacgccca ttgtggtctc
     6961 ctcgcccttg ctcaccatgg tggcggctag cataacttcg tataaagtat cctatacgaa
     7021 gttatttgcc ttaacccaga aattatcact gttattcttt agaatggtgc aaagaataac
     7081 ttcgtataat gtatgctata cgaagttatg aattcgatat caagcttatc gataatcaac
     7141 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     7201 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     7261 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     7321 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     7381 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     7441 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     7501 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     7561 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     7621 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     7681 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc
     7741 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
     7801 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac
     7861 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     7921 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct
     7981 accacacaca aggctacttc cctgattggc agaactacac accagggcca gggatcagat
     8041 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag
     8101 aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg
     8161 acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg
     8221 cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg
     8281 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     8341 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     8401 tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact
     8461 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     8521 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     8581 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     8641 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     8701 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     8761 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     8821 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     8881 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     8941 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     9001 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     9061 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     9121 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     9181 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     9241 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     9301 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     9361 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     9421 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     9481 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     9541 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
     9601 gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
     9661 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
     9721 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
     9781 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
     9841 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
     9901 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
     9961 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc
    10021 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
    10081 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
    10141 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    10201 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
    10261 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
    10321 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
    10381 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
    10441 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
    10501 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    10561 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    10621 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    10681 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    10741 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    10801 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    10861 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    10921 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    10981 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    11041 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    11101 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
    11161 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    11221 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    11281 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    11341 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    11401 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    11461 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    11521 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    11581 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    11641 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    11701 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    11761 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    11821 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    11881 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    11941 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    12001 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    12061 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    12121 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    12181 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    12241 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    12301 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    12361 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    12421 tccccgaaaa gtgccacctg ac
//
