LOCUS       sequence-142210-         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for SICLOPPS-mediated protein circularization using the 
            gp41-1 intein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Waldhauer MC, Schmitz SN, Ahlmann-Eltze C, Gleixner JG, Schmelas CC,
            Huhn AG, Bunne C, Buscher M, Horn M, Klughammer N, Kreft J, Schafer 
            E, Bayer PA, Kramer SG, Neugebauer J, Wehler P, Mayer MP, Eils R, Di
            Ventura B
  TITLE     Backbone circularization of Bacillus subtilis family 11 xylanase 
            increases its thermostability and its resistance against 
            aggregation.
  JOURNAL   Mol Biosyst. 2015 Nov 10;11(12):3231-43. doi: 10.1039/c5mb00341e.
  PUBMED    26434634
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2015-11-10- 10"; volume: "11"; issue: "12"; 
            pages: "3231-43"
COMMENT     Sequence Label: pCIRCgp41-1
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..32
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(36..79)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     87..106
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        87..105
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    106..130
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    133..154
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     RBS             161..183
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    408..429
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(444..449,450..467,468..474)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    482..498
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             516..527
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 attaacctat aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc
       61 tttcgctaag gatgatttct ggaatttaat acgactcact ataggggaat tgtgagcgga
      121 taacaattcc ccgaattcgc ggccgcttct agagaataat tttgtttaac tttaagaagg
      181 agatactaga tgatgctgaa aaaaatcctg aaaatcgaag agctggatga acgtgaactg
      241 atcgatattg aggtgtccgg taaccacctg ttttacgcta acgatattct gacccacaac
      301 agagacccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      361 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      421 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      481 attgtgagcg gataacaatt tcacacatac tagagaaaga ggagaaatac tagatggctt
      541 cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta
      601 acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga
      661 ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc
      721 cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc ccggactacc
      781 tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg
      841 gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc tacaaagtta
      901 aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaa
//
