LOCUS       sequence-142212-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For generating mRNA for zebrafish injection of cytoplasmic 
            fluorogen-activating protein (FAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   He J, Wang Y, Missinato MA, Onuoha E, Perkins LA, Watkins SC, St 
            Croix CM, Tsang M, Bruchez MP
  TITLE     A genetically targetable near-infrared photosensitizer.
  JOURNAL   Nat Methods. 2016 Mar;13(3):263-268. doi: 10.1038/nmeth.3735. Epub 
            2016 Jan 25.
  PUBMED    26808669
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3735"; journalName: "Nat Methods"; date: "2016-03"; 
            volume: "13"; issue: "3"; pages: "263-268"
COMMENT     Sequence Label: pCS2+ MBIC5-mCer3
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(67..786)
                     /codon_start=1
                     /product="fluorogen-activating protein composed of a dimer 
                     of the L5-MG single-chain antibody fragment (Szent-Gyorgyi 
                     et al., 2008)"
                     /label=FAP-alpha-2
                     /note="binds malachite green derivatives to produce red 
                     fluorescence"
                     /translation="QAVVTQEPSVTVSPGGTVILTCGSGTGAVTSGHYANWFQQKPGQA
                     PRALIFDTDKKYSWTPGRFSGSLLGAKAALTISDAQPEDEAEYYCSLSDVDGYLFGGGT
                     QLTVLSGGGGSGGGGSGGGGSGGGGSQAVVTQEPSVTVSPGGTVILTCGSGTGAVTSGH
                     YANWFQQKPGQAPRALIFDTDKKYSWTPGRFSGSLLGAKAALTISDAQPEDEAEYYCSL
                     SDVDGYLFGGGTQLTVLS"
ORIGIN
        1 tgctaccata ccggtgctgc caccgccacc agacccaccg cctcccatat gggccagacc
       61 ggccgcggag aggacggtca gctgggtgcc tcctccgaac agataaccgt caacgtcgga
      121 gagcgaacag taatactcag cctcatcttc aggctgcgca tccgagatgg tcagggcagc
      181 cttggcccca aggagggagc ctgagaatcg gccaggggtc caggaatact tcttgtcggt
      241 gtcaaatata agtgccctgg gggcttggcc aggcttctgc tggaaccagt tggcataatg
      301 accactggtg acagctccag tgccggagcc acaagtgaga atgactgtcc ctcctgggga
      361 cacagtcact gacggctcct gagtcaccac agcctgagaa ccgccgcctc cggaaccacc
      421 accgccgctg ccaccgccac cagagccgcc accaccggac agaaccgtca gttgtgttcc
      481 accgccaaac aagtagccat ccacgtcgct aagagaacag taatactcgg cttcgtcctc
      541 aggctgagca tctgatattg tcaaagctgc ctttgctccc agcaaagatc cagaaaatct
      601 acctggggtc caagaatact tcttatcagt atcaaaaatc aaagctcttg gagcttgtcc
      661 tggtttttgt tgaaaccaat ttgcataatg accagatgtg acggcacccg taccacttcc
      721 acaagtaagt ataacagtac cacctgggct aacggtaaca ctaggttctt gggtaacgac
      781 ggcctgcagg ccagaaaggc cgaattccat ggagaagaca ttggaagagc ctctttgagc
      841 agttttcccc
//
