LOCUS       sequence-142215-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses hM4Dnrxn and a red fluorescent label from a 
            Cre-dependent virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Stachniak TJ, Ghosh A, Sternson SM
  TITLE     Chemogenetic synaptic silencing of neural circuits localizes a 
            hypothalamus-->midbrain pathway for feeding behavior.
  JOURNAL   Neuron. 2014 May 21;82(4):797-808. doi: 
            10.1016/j.neuron.2014.04.008. Epub 2014 Apr 24.
  PUBMED    24768300
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2014.04.008"; journalName: "Neuron"; date: 
            "2014-05-21- 21"; volume: "82"; issue: "4"; pages: "797-808"
COMMENT     Sequence Label: AA0250-mCherry-2a-hM4D-nrxn1a rev
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(101..154)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ataccatttc tactgtttcg tagcgattgt gcgaggacga cgtcacgagc cgtaccgact
       61 gattaccgga gctcccattg acaggcgtga agttcgccat aggtcccgga ttctcttcga
      121 catccccaca cgtcaaaagg ctcccgcgtc cttctgctct gctagcctta tacaactcat
      181 ccattccccc ggtcgaatgt cgcccctccg ccctttcgta ctgttccacg atcgtataat
      241 cctcgttatg gcttgtaatg tcgagtttga tattcacatt gtaggcaccc ggcaactgca
      301 ccggcttctt cgccttatac gttgtcttga cttcggcgtc gtagtgtcca ccgtctttca
      361 acttcaatct ctgtttgatc tcccctttga gtgctccatc ttcgggatac atccgctccg
      421 acgacgcctc ccaacccatc gtctttttct gcatgactgg accgtccgag gggaaattag
      481 tgcctctgag tttcaccttg tagataaact cgccgtcttg gagggacgag tcttgcgtca
      541 ctgtcactac ccctccatcc tcaaaattca tcactcgctc ccatttgaag ccctcgggaa
      601 acgacagttt aagatagtct ggaatgtccg cgggatgctt cacatacgct ttggacccgt
      661 acataaactg cggtgaaagg atgtcccacg cgaaaggcaa cggtcctcct ttggtgactt
      721 tcagcttggc tgtctgtgtc ccctcgtagg ggcgtccctc accctctccc tcgatctcaa
      781 actcgtgccc gttgacgctg ccttccatgt gcaccttaaa ccgca
//
