LOCUS       sequence-142222-         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid containing tetracycline resistance gene cassette flanked by 
            multiple restriction sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Ledermann R, Strebel S, Kampik C, Fischer HM
  TITLE     Versatile vectors for efficient mutagenesis of Bradyrhizobium 
            diazoefficiens and other Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2016 Feb 26. pii: AEM.04085-15.
  PUBMED    26921431
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2016 Feb 26. pii: AEM.04085-15."
COMMENT     Sequence Label: pRGD-TcR
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(306..323)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(306..322)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(315..337)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gcggctctta ccagcctaac ttcgatcatt ggaccgctga tcgtcacggc gatttatgcc
       61 gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc
      121 tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc
      181 ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga
      241 gaactgtgaa tgcgcaccta gatcttcgaa cgatactagt catatgaatt cggtaccagc
      301 ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
      361 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
      421 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
      481 ctccttacgc atctgtgcgg tatttcacac cgcataactg gctgctattg ggcgaagtgc
      541 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
      601 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
      661 aacatcgcat cgagcgagca cgtactc
//
