LOCUS       sequence-142224-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein Quantitation Reporter (PQR) to quantify a protein of 
            interest in insect cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Lo CA, Kays I, Emran F, Lin TJ, Cvetkovska V, Chen BE
  TITLE     Quantification of Protein Levels in Single Living Cells.
  JOURNAL   Cell Rep. 2015 Dec 22;13(11):2634-44. doi: 
            10.1016/j.celrep.2015.11.048. Epub 2015 Dec 10.
  PUBMED    26686644
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.11.048"; journalName: "Cell Rep"; date: 
            "2015-12-22- 22"; volume: "13"; issue: "11"; pages: "2634-44"
COMMENT     Sequence Label: pJFRC7-RFPnls-PQRv2-mCD8:GFP
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             82..102
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             106..126
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             130..150
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             172..225
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             226..891
                     /codon_start=1
                     /product="mouse lymphocyte antigen CD8 alpha chain"
                     /label=mCD8
                     /translation="MASPLTRFLSLNLLLLGESIILGSGEAKPQAPELRIFPKKMDAEL
                     GQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSA
                     MRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSP
                     VHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIYIWAPLAGICVALLLSLIITLICYHSR
                     "
ORIGIN
        1 gctgtggcca gatactgcga cctccctagc aaactggggc acaaacttaa tggcatggac
       61 gagctgtaca agctcgagga cccaaagaag aagcggaaag tggatccaaa gaaaaagcgc
      121 aaggtggacc ctaagaagaa acggaaggtg accggtatcg atgggtccgg ggaaggtcgt
      181 ggtagtctac taacgtgtgg tgatgtagaa gaaaatcctg gacctatggc ctcaccgttg
      241 acccgctttc tgtcgctgaa cctgctgctg ctgggtgagt cgattatcct ggggagtgga
      301 gaagctaagc cacaggcacc cgaactccga atctttccaa agaaaatgga cgccgaactt
      361 ggtcagaagg tggacctggt atgtgaagtg ttggggtccg tttcgcaagg atgctcttgg
      421 ctcttccaga actccagctc caaactcccc cagcccacct tcgttgtcta tatggcttca
      481 tcccacaaca agataacgtg ggacgagaag ctgaattcgt cgaaactgtt ttctgccatg
      541 agggacacga ataataagta cgttctcacc ctgaacaagt tcagcaagga aaacgaaggc
      601 tactatttct gctcagtcat cagcaactcg gtgatgtact tcagttctgt cgtgccagtc
      661 cttcagaaag tgaactctac tactaccaag ccagtgctgc gaactccctc acctgtgcac
      721 cctaccggga catctcagcc ccagagacca gaagattgtc ggccccgtgg ctcagtgaag
      781 gggaccggat tggacttcgc ctgtgatatt tacatctggg cacccttggc cggaatctgc
      841 gtggcccttc tgctgtcctt gatcatcact ctcatctgct accacagccg cggatcc
//
