LOCUS       sequence-142225-         677 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid containing tetracycline resistance gene cassette flanked by 
            multiple restriction sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Ledermann R, Strebel S, Kampik C, Fischer HM
  TITLE     Versatile vectors for efficient mutagenesis of Bradyrhizobium 
            diazoefficiens and other Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2016 Feb 26. pii: AEM.04085-15.
  PUBMED    26921431
REFERENCE   2  (bases 1 to 677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2016 Feb 26. pii: AEM.04085-15."
COMMENT     Sequence Label: pRGD-TcR
FEATURES             Location/Qualifiers
     source          1..677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     186..205
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(605..610,611..627,628..633))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc
       61 gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct
      121 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc
      181 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg
      241 gccacgatgc gtccggcgta gaggatccac aggacgggtg tggtcgccat gatcgcgtag
      301 tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac
      361 agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg
      421 ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc
      481 ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc
      541 gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta
      601 ccgcattaaa gcttatcgat gataagctgt caaacatgag aattatcact agtgaatcat
      661 cgatggtacc atatgtt
//
