LOCUS       sequence-142227-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid containing streptomycin resistance gene cassette flanked by 
            multiple restriction sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Ledermann R, Strebel S, Kampik C, Fischer HM
  TITLE     Versatile vectors for efficient mutagenesis of Bradyrhizobium 
            diazoefficiens and other Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2016 Feb 26. pii: AEM.04085-15.
  PUBMED    26921431
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2016 Feb 26. pii: AEM.04085-15."
COMMENT     Sequence Label: pRGD-SmR
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(501..518)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(501..517)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(510..532)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(726..745)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc
       61 accaaggtag tcggcaaata atgtctaaca attcgttcaa gccgacgccg cttcgcggcg
      121 cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca aactatcagg
      181 tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata
      241 tatcatgaaa ggctggcttt ttcttgttat cgcaatagtt ggcgaagtaa tcgcaacatc
      301 cgcattaaaa tctagcgagg gctttactaa gctgatccgg tggatgacct tttgaatgac
      361 ctttaataga ttatattact aattaattgg ggaccctaga ggtccccttt tttattttaa
      421 aaattttttc acaaaacggt ttacaagcat aaagctatca ctagtgaatc atcgatggta
      481 ccatatgttc gaagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
      541 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
      601 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
      661 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
      721 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
      781 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg ctta
//
