LOCUS       sequence-142230-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mCherry-encoding vector for markerless deletion mutagenesis in 
            Bradyrhizobium diazoefficiens and other Alphaproteobacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Ledermann R, Strebel S, Kampik C, Fischer HM
  TITLE     Versatile vectors for efficient mutagenesis of Bradyrhizobium 
            diazoefficiens and other Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2016 Feb 26. pii: AEM.04085-15.
  PUBMED    26921431
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2016 Feb 26. pii: AEM.04085-15."
COMMENT     Sequence Label: pTETSIX
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     211..228
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(597..616)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
       61 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
      121 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
      181 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
      241 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcag
      301 tcaagatcta agcttcaatt ccttgtacag ctcgtccatg ccgccggtgg agtggcggcc
      361 ctcggcgcgt tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa
      421 cttgatgttg acgttgtagg cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt
      481 cttgacctca gcgtcgtagt ggccgccgtc cttcagcttc agcctctgct tgatctcgcc
      541 cttcagggcg ccgtcctcgg ggtacatccg ctcggaggag gcctcccagc ccatggtctt
      601 cttctgcatt acggggccgt cggaggggaa gttggtgccg cgcagcttca ccttgtagat
      661 gaactcgccg tcctgcaggg aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa
      721 gttcatcacg cgctcccact tgaagccctc ggggaaggac agcttcaagt agtcggggat
      781 gtcggcgggg tgcttcacgt aggccttgga gccgtacatg aactgagggg acaggatgtc
      841 ccaggcga
//
