LOCUS       sequence-142235-        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express fluorescent calcium indicator GCaMP3fast in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Helassa N, Zhang XH, Conte I, Scaringi J, Esposito E, Bradley J, 
            Carter T, Ogden D, Morad M, Torok K
  TITLE     Fast-Response Calmodulin-Based Fluorescent Indicators Reveal Rapid 
            Intracellular Calcium Dynamics.
  JOURNAL   Sci Rep. 2015 Nov 3;5:15978. doi: 10.1038/srep15978.
  PUBMED    26527405
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-11-3"; volume: "5:15978. doi"; pages: " 
            10.1038/srep15978"
COMMENT     Sequence Label: pCMV-GCaMP3fast
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      64..73
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             79..96
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             100..132
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(136..144,145..159)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      166..175
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             187..243
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKYNKTGHAVRAIGRLSS"
     primer_bind     456..477
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(583..604)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(844..863)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat
       61 ctcgccacca tgggttctca tcatcatcat catcatggta tggctagcat gactggtgga
      121 cagcaaatgg gtcgggatct gtacgacgat gacgataagg atctcgccac catggtcgac
      181 tcatcacgtc gtaagtacaa taagacaggt cacgcagtca gagctatagg tcggctgagc
      241 tcactcgaga acgtctatat caaggccgac aagcagaaga acggcatcaa ggcgaacttc
      301 aagatccgcc acaacatcga ggacggcggc gtgcagctcg cctaccacta ccagcagaac
      361 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cgtccagtcc
      421 aaactttcga aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
      481 gccgccggga tcactctcgg catggacgag ctgtacaagg gcggtaccgg agggagcatg
      541 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
      601 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggtg agggcgatgc cacctacggc
      661 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
      721 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
      781 cacgacttct tcaagtccgc catgcccgaa ggctacatcc aggagcgcac catcttcttc
      841 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
      901 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
      961 ctggagtaca acacgcgtga ccaactgact gaagagcaga tcgcagagtt
//
