LOCUS       sequence-142236-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express fluorescent calcium indicator GCaMP3fast in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Helassa N, Zhang XH, Conte I, Scaringi J, Esposito E, Bradley J, 
            Carter T, Ogden D, Morad M, Torok K
  TITLE     Fast-Response Calmodulin-Based Fluorescent Indicators Reveal Rapid 
            Intracellular Calcium Dynamics.
  JOURNAL   Sci Rep. 2015 Nov 3;5:15978. doi: 10.1038/srep15978.
  PUBMED    26527405
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-11-3"; volume: "5:15978. doi"; pages: " 
            10.1038/srep15978"
COMMENT     Sequence Label: pCMV-GCaMP3fast
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    131..252
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(142..161)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     196..215
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(374..817)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFAKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     primer_bind     933..952
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
       61 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
      121 atttacgcct taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
      181 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
      241 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
      301 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcta
      361 gagtcgcggc cgctcacttc gctgtcatca tttgtacaaa ctcttcgtag tttacctgac
      421 catccccatc gatgtctgct tccctgatca tttcatcaac ctcttcatct gttaacttct
      481 ctccaaggtt tgtcatcacg tggcgaagct ctgctgcact gatgtagcca ttgccatcct
      541 tagcaaacac acggaacgct tctctaattt cttcttcact gtctgtgtct ttcatttttc
      601 ttgccatcat tgtcaggaac tcagggaagt cgattgtgcc atcaccgtcg gcatctactt
      661 cattgatcat gtcctgcagc tctgcttctg tggggttctg ccccagagac cgcatcaccg
      721 tccccagctc cttggttgtt attgtcccat ccccgtcctt gtcaaatagg gagaaagcct
      781 ctttaaattc tgcgatctgc tcttcagtca gttggtcacg cgtgttgtac tccagcttgt
      841 gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca
      901 gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga
      961 aga
//
