LOCUS       sequence-142238-         455 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An adaption on the RCAS/tv-a somatic cell gene transfer system, for 
            use in combination with an existing Cas9 background in the 
            cell/mouse of interest. Depositors: Jane Fraser/Noor Gammoh.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Fraser J, Simpson J, Fontana R, Kishi-Itakura C, Ktistakis NT, 
            Gammoh N
  TITLE     Targeting of early endosomes by autophagy facilitates EGFR recycling
            and signalling.
  JOURNAL   EMBO Rep. 2019 Oct 4;20(10):e47734. doi: 10.15252/embr.201947734. 
            Epub 2019 Aug 26.
  PUBMED    31448519
REFERENCE   2  (bases 1 to 455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep.";
            date: "2019-10-4"; volume: "20(10):e47734. doi"; pages: " 
            10.15252/embr.201947734. Epub 2019 Aug 26"
COMMENT     Sequence Label: RCAS-sgATG7
FEATURES             Location/Qualifiers
     source          1..455
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        70..318
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     70..90
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     241..260
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        339..414
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(426..450)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgtacaaaaa agcaggcttt aaaggaacca attcagtcga ctggatccgg taccaaggtc
       61 gggcaggaag agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
      121 gttagagaga taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg
      181 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
      241 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
      301 tggaaaggac gaaacaccgg aaacttgttg aggagcatgt tttagagcta gaaatagcaa
      361 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
      421 tctagaccca gctttcttgt acaaagttgg catta
//
