LOCUS       sequence-142262-        9956 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral expression plasmid for human NFATc1/bC with C-terminal 
            BirA-biotinylation signal and TEV celavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9956)
  AUTHORS   Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, 
            Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel 
            H, Radbruch A, Krause E, Baumgrass R
  TITLE     Identification of Novel Nuclear Factor of Activated T Cells 
            (NFAT)-Associated Proteins in T cells.
  JOURNAL   J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
  PUBMED    27637333
REFERENCE   2  (bases 1 to 9956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Sep 16. pii: jbc.M116.739326."
COMMENT     Sequence Label: pMIG-hNFATc1/bC - AVITEV
FEATURES             Location/Qualifiers
     source          1..9956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             43..560
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    624..965
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1032..1448
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1378..1400
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1416..1432
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             4281..4301
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4314..4358
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     primer_bind     4397..4413
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4398..4414
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4433..4999
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4449..5001
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4593..4610)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4820..4839
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5013..5015,5016..5018,5019..5732)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5013..5015,5016..5018,5019..5729)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5058..5079)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5319..5338)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5666..5687
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5823..6338
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(6507..6523)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6507..6523)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6520..6542)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6531..6547
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6555..6561,6562..6579,6580..6585))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6600..6621
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6738..6755)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6909..7497)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6989..7008)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7668..8459,8460..8528))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8291..8310
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8529..8633)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8701..8719
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8757..8779)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8879..8898
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8921..8941)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8926..9255
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9106..9241
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9168..9187
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9303..9350
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     9544..9566
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9558..9575
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9559..9575
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9778..9797
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg
       61 taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa tacataactg
      121 agaatagaga agttcagatc aaggttagga acagagagac agcagaatat gggccaaaca
      181 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga
      241 tgcggtcccg ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg
      301 acctgaaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
      361 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc
      421 agtcctccga tagactgcgt cgcccgggta cccgtattcc caataaagcc tcttgctgtt
      481 tgcatccgaa tcgtggactc gctgatcctt gggagggtct cctcagattg attgactgcc
      541 cacctcgggg gtctttcatt tggaggttcc accgagattt ggagacccct gcctagggac
      601 caccgacccc cccgccggga ggtaagctgg ccagcggtcg tttcgtgtct gtctctgtct
      661 ttgtgcgtgt ttgtgccggc atctaatgtt tgcgcctgcg tctgtactag ttagctaact
      721 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc
      781 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt
      841 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac
      901 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc
      961 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt
     1021 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag
     1081 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct
     1141 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc
     1201 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
     1261 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
     1321 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
     1381 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
     1441 taggcgccgg aattagatct gcggccgcgg atttaaatcc caccatgacg gggctggagg
     1501 accaggagtt cgacttcgag ttcctcttcg agtttaacca gcgcgacgag ggcgccgccg
     1561 cggccgcccc agaacactat ggctatgcat cctccaacgt cagccccgcc ctgccgctcc
     1621 ccacggcgca ctccaccctg ccggccccgt gccacaacct tcagacctcc acaccgggca
     1681 tcatcccgcc ggcggatcac ccctcggggt acggagcagc tttggacggt gggcccgcgg
     1741 gctacttcct ctcctccggc cacaccaggc ctgatggggc ccctgccctg gagagtcctc
     1801 gcatcgagat aacctcgtgc ttgggcctgt accacaacaa taaccagttt ttccacgatg
     1861 tggaggtgga agacgtcctc cctagctcca aacggtcccc ctccacggcc acgctgagtc
     1921 tgcccagcct ggaggcctac agagacccct cgtgcctgag cccggccagc agcctgtcct
     1981 cccggagctg caactcagag gcctcctcct acgagtccaa ctactcgtac ccgtacgcgt
     2041 ccccccagac gtcgccatgg cagtctccct gcgtgtctcc caagaccacg gaccccgagg
     2101 agggctttcc ccgcgggctg ggggcctgca cactgctggg ttccccgcgg cactccccct
     2161 ccacctcgcc ccgcgccagc gtcactgagg agagctggct gggtgcccgc tcctccagac
     2221 ccgcgtcccc ttgcaacaag aggaagtaca gcctcaacgg ccggcagccg ccctactcac
     2281 cccaccactc gcccacgccg tccccgcacg gctccccgcg ggtcagcgtg accgacgact
     2341 cgtggttggg caacaccacc cagtacacca gctcggccat cgtggccgcc atcaacgcgc
     2401 tgaccaccga cagcagcctg gacctgggag atggcgtccc tgtcaagtcc cgcaagacca
     2461 ccctggagca gccgccctca gtggcgctca aggtggagcc cgtcggggag gacctgggca
     2521 gccccccgcc cccggccgac ttcgcgcccg aagactactc ctctttccag cacatcagga
     2581 agggcggctt ctgcgaccag tacctggcgg tgccgcagca cccctaccag tgggcgaagc
     2641 ccaagcccct gtcccctacg tcctacatga gcccgaccct gcccgccctg gactggcagc
     2701 tgccgtccca ctcaggcccg tatgagcttc ggattgaggt gcagcccaag tcccaccacc
     2761 gagcccacta cgagacggag ggcagccggg gggccgtgaa ggcgtcggcc ggaggacacc
     2821 ccatcgtgca gctgcatggc tacttggaga atgagccgct gatgctgcag cttttcattg
     2881 ggacggcgga cgaccgcctg ctgcgcccgc acgccttcta ccaggtgcac cgcatcacag
     2941 ggaagaccgt gtccaccacc agccacgagg ccatcctctc caacaccaaa gtcctggaga
     3001 tcccactcct gccggagaac agcatgcgag ccgtcattga ctgtgccgga atcctgaaac
     3061 tcagaaactc cgacattgaa cttcggaaag gagagacgga catcgggagg aagaacacac
     3121 gggtacggct ggtgttccgc gttcacgtcc cgcaacccag cggccgcacg ctgtccctgc
     3181 aggtggcctc caaccccatc gaatgctccc agcgctcagc tcaggagctg cctctggtgg
     3241 agaagcagag cacggacagc tatccggtcg tgggcgggaa gaagatggtc ctgtctggcc
     3301 acaacttcct gcaggactcc aaggtcattt tcgtggagaa agccccagat ggccaccatg
     3361 tctgggagat ggaagcgaaa actgaccggg acctgtgcaa gccgaattct ctggtggttg
     3421 agatcccgcc atttcggaat cagaggataa ccagccccgt tcacgtcagt ttctacgtct
     3481 gcaacgggaa gagaaagcga agccagtacc agcgtttcac ctaccttccc gccaacgttc
     3541 caattataaa aacagaaccc actgatgatt atgagcctgc tccaacctgt ggaccggtga
     3601 gccaggggtt aagtcctctc ccaagaccat actacagcca gcagctcgcg atgccacccg
     3661 accccagctc ctgcctcgtg gccggcttcc cgccctgtcc gcagagaagc accctgatgc
     3721 cagcggcccc tggcgtgagc cccaagctcc acgacctttc tcccgctgcc tacaccaagg
     3781 gcgttgccag cccgggccac tgtcacctcg gactcccgca gccggccgga gaggcccccg
     3841 ccgtccagga cgtgcccagg ccagtggcca cgcaccccgg ctcgcccggg cagccacccc
     3901 cggccctgct gccacagcag gtgagtgcgc ctccaagcag tagctgcccc cctggtctcg
     3961 aacactcgct ctgccccagc agcccctctc ctccactccc gcctgccacc caagagccga
     4021 cctgcctgca gccctgcagc ccagcgtgcc cgcccgccac gggccgcccg cagcacctgc
     4081 cgtccacggt ccgcagggac gagtctccga ctgccgggcc acggctgctg ccagaggtgc
     4141 atgaggacgg tagtcctaat ttggccccta ttcctgtaac ggtcaagcga gagcctgaag
     4201 agttggacca gttgtacctg gatgacgtaa atgaaataat acgaaatgac ctctccagca
     4261 cgagcaccca ctcctacgta gagaacctgt acttccaggg cgttaacggc ggtggcctga
     4321 acgatatctt cgaggcacag aaaatcgaat ggcatgaggt taactgagcg atcgcttata
     4381 agcggccgcg tcgaggtcga ggtcgacggt atcgataagc ttgatatcga attccgcccc
     4441 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     4501 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     4561 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     4621 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     4681 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     4741 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     4801 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     4861 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     4921 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     4981 ttgaaaaaca cgatgataat atggccacaa ccatggtgag caagggcgag gagctgttca
     5041 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     5101 tgtctggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     5161 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     5221 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     5281 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     5341 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     5401 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     5461 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     5521 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     5581 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     5641 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     5701 tcactctcgg catggacgag ctgtacaagt aagaattcct gcagcccggg ggatccgtcg
     5761 acctgcagcc aagcttatcg ataaaataaa agattttatt tagtctccag aaaaaggggg
     5821 gaatgaaaga ccccacctgt aggtttggca agctagctta attaacgcca ttttgcaagg
     5881 catggaaaat acataactga gaatagagaa gttcagatca aggttaggaa cagagagaca
     5941 gcagaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     6001 agaacagatg gtccccagat gcggtcccgc cctcagcagt ttctagagaa ccatcagatg
     6061 tttccagggt gccccaagga cctgaaaatg accctgtgcc ttatttgaac taaccaatca
     6121 gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca
     6181 acccctcact cggcgcgcca gtcctccgat agactgcgtc gcccgggtac ccgtgtatcc
     6241 aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc
     6301 tctgagtgat tgactacccg tcagcggggg tctttcatgg gtaacagttt cttgaagttg
     6361 gagaacaaca ttctgagggt aggagtcgaa tattaagtaa tcctgactca attagccact
     6421 gttttgaatc cacatactcc aatactcctg aaatagttca ttatggacag cgcagaagag
     6481 ctggggagaa ttaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     6541 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6601 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     6661 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     6721 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     6781 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     6841 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6901 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6961 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7021 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7081 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7141 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7201 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7261 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7321 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     7381 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7441 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7501 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7561 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7621 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7681 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     7741 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7801 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     7861 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7921 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7981 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8041 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8101 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8161 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8221 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     8281 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8341 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8401 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8461 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8521 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     8581 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     8641 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     8701 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     8761 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     8821 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     8881 gcatcagagc agattgtact gagagtgcac catatgctgt ggaatgtgtg tcagttaggg
     8941 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     9001 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     9061 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     9121 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     9181 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     9241 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
     9301 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
     9361 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     9421 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     9481 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     9541 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctcgctctc ccttatgcga
     9601 ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag
     9661 gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat
     9721 acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt
     9781 gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat
     9841 gcgtccggcg tagaggatcg attagtccaa tttgttaaag acaggatatc agtggtccag
     9901 gctctagttt tgactcaaca atatcaccag ctgaagccta tagagtacga gccata
//
