LOCUS       sequence-142263-        9320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral expression plasmid for human NFATc1/aA with N-terminal 
            BirA-biotinylation signal and TEV celavage site .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9320)
  AUTHORS   Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, 
            Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel 
            H, Radbruch A, Krause E, Baumgrass R
  TITLE     Identification of Novel Nuclear Factor of Activated T Cells 
            (NFAT)-Associated Proteins in T cells.
  JOURNAL   J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
  PUBMED    27637333
REFERENCE   2  (bases 1 to 9320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Sep 16. pii: jbc.M116.739326."
COMMENT     Sequence Label: pMIG-AVITEV- hNFATc1/aA
FEATURES             Location/Qualifiers
     source          1..9320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             43..560
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    624..965
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1032..1448
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1378..1400
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1416..1432
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1479..1488
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1497..1541
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             1548..1568
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     3761..3777
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3762..3778
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3797..4363
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3813..4365
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3957..3974)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4184..4203
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4377..4379,4380..4382,4383..5096)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4377..4379,4380..4382,4383..5093)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4422..4443)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4683..4702)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5030..5051
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5187..5702
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(5871..5887)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5871..5887)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5884..5906)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5895..5911
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5919..5925,5926..5943,5944..5949))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5964..5985
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6102..6119)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6273..6861)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6353..6372)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7032..7823,7824..7892))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7655..7674
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7893..7997)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8065..8083
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8121..8143)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8243..8262
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8285..8305)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8290..8619
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8470..8605
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8532..8551
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8667..8714
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     8908..8930
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8922..8939
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8923..8939
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9142..9161
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg
       61 taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa tacataactg
      121 agaatagaga agttcagatc aaggttagga acagagagac agcagaatat gggccaaaca
      181 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga
      241 tgcggtcccg ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg
      301 acctgaaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
      361 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc
      421 agtcctccga tagactgcgt cgcccgggta cccgtattcc caataaagcc tcttgctgtt
      481 tgcatccgaa tcgtggactc gctgatcctt gggagggtct cctcagattg attgactgcc
      541 cacctcgggg gtctttcatt tggaggttcc accgagattt ggagacccct gcctagggac
      601 caccgacccc cccgccggga ggtaagctgg ccagcggtcg tttcgtgtct gtctctgtct
      661 ttgtgcgtgt ttgtgccggc atctaatgtt tgcgcctgcg tctgtactag ttagctaact
      721 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc
      781 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt
      841 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac
      901 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc
      961 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt
     1021 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag
     1081 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct
     1141 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc
     1201 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
     1261 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
     1321 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
     1381 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
     1441 taggcgccgg aattagatct gcggccgcgg atttaaatgc caccatggtt aacagcggcc
     1501 tgaacgatat cttcgaggca cagaaaatcg aatggcatga ggttaacgag aacctgtact
     1561 tccagggcat gcattacgta atgccaagca ccagctttcc agtcccttcc aagtttccac
     1621 ttggccctgc ggctgcggtc ttcgggagag gagaaacttt ggggcccgcg ccgcgcgccg
     1681 gcggcaccat gaagtcagcg gaggaagaac actatggcta tgcatcctcc aacgtcagcc
     1741 ccgccctgcc gctccccacg gcgcactcca ccctgccggc cccgtgccac aaccttcaga
     1801 cctccacacc gggcatcatc ccgccggcgg atcacccctc ggggtacgga gcagctttgg
     1861 acggtgggcc cgcgggctac ttcctctcct ccggccacac caggcctgat ggggcccctg
     1921 ccctggagag tcctcgcatc gagataacct cgtgcttggg cctgtaccac aacaataacc
     1981 agtttttcca cgatgtggag gtggaagacg tcctccctag ctccaaacgg tccccctcca
     2041 cggccacgct gagtctgccc agcctggagg cctacagaga cccctcgtgc ctgagcccgg
     2101 ccagcagcct gtcctcccgg agctgcaact cagaggcctc ctcctacgag tccaactact
     2161 cgtacccgta cgcgtccccc cagacgtcgc catggcagtc tccctgcgtg tctcccaaga
     2221 ccacggaccc cgaggagggc tttccccgcg ggctgggggc ctgcacactg ctgggttccc
     2281 cgcagcactc cccctccacc tcgccccgcg ccagcgtcac tgaggagagc tggctgggtg
     2341 cccgctcctc cagacccgcg tccccttgca acaagaggaa gtacagcctc aacggccggc
     2401 agccgcccta ctcaccccac cactcgccca cgccgtcccc gcacggctcc ccgcgggtca
     2461 gcgtgaccga cgactcgtgg ttgggcaaca ccacccagta caccagctcg gccatcgtgg
     2521 ccgccatcaa cgcgctgacc accgacagca gcctggacct gggagatggc gtccctgtca
     2581 agtcccgcaa gaccaccctg gagcagccgc cctcagtggc gctcaaggtg gagcccgtcg
     2641 gggaggacct gggcagcccc ccgcccccgg ccgacttcgc gcccgaagac tactcctctt
     2701 tccagcacat caggaagggc ggcttctgcg accagtacct ggcggtgccg cagcacccct
     2761 accagtgggc gaagcccaag cccctgtccc ctacgtccta catgagcccg accctgcccg
     2821 ccctggactg gcagctgccg tcccactcag gcccgtatga gcttcggatt gaggtgcagc
     2881 ccaagtccca ccaccgagcc cactacgaga cggagggcag ccggggggcc gtgaaggcgt
     2941 cggccggagg acaccccatc gtgcagctgc atggctactt ggagaatgag ccgctgatgc
     3001 tgcagctttt cattgggacg gcggacgacc gcctgctgcg cccgcacgcc ttctaccagg
     3061 tgcaccgcat cacagggaag accgtgtcca ccaccagcca cgaggctatc ctctccaaca
     3121 ccaaagtcct ggagatccca ctcctgccgg agaacagcat gcgagccgtc attgactgtg
     3181 ccggaatcct gaaactcaga aactccgaca ttgaacttcg gaaaggagag acggacatcg
     3241 ggaggaagaa cacacgggta cggctggtgt tccgcgttca cgtcccgcaa cccagcggcc
     3301 gcacgctgtc cctgcaggtg gcctccaacc ccatcgaatg ctcccagcgc tcagctcagg
     3361 agctgcctct ggtggagaag cagagcacgg acagctatcc ggtcgtgggc gggaagaaga
     3421 tggtcctgtc tggccacaac ttcctgcagg actccaaggt cattttcgtg gagaaagccc
     3481 cagatggcca ccatgtctgg gagatggaag cgaaaactga ccgggacctg tgcaagccga
     3541 attctctggt ggttgagatc ccgccatttc ggaatcagag gataaccagc cccgttcacg
     3601 tcagtttcta cgtctgcaac gggaagagaa agcgaagcca gtaccagcgt ttcacctacc
     3661 ttcccgccaa cggtaacgcc atctttctaa ccgtaagccg tgaacatgag cgcgtggggt
     3721 gctttttcta agcgatcgct tataagcggc cgcgtcgagg tcgaggtcga cggtatcgat
     3781 aagcttgata tcgaattccg cccccccccc taacgttact ggccgaagcc gcttggaata
     3841 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     3901 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     3961 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     4021 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     4081 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     4141 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     4201 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     4261 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     4321 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatgg
     4381 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     4441 acgtaaacgg ccacaagttc agcgtgtctg gcgagggcga gggcgatgcc acctacggca
     4501 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     4561 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     4621 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     4681 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     4741 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     4801 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     4861 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     4921 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     4981 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     5041 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagaat
     5101 tcctgcagcc cgggggatcc gtcgacctgc agccaagctt atcgataaaa taaaagattt
     5161 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
     5221 cttaattaac gccattttgc aaggcatgga aaatacataa ctgagaatag agaagttcag
     5281 atcaaggtta ggaacagaga gacagcagaa tatgggccaa acaggatatc tgtggtaagc
     5341 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc ccgccctcag
     5401 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa aatgaccctg
     5461 tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc
     5521 ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcctc cgatagactg
     5581 cgtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc
     5641 tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc
     5701 atgggtaaca gtttcttgaa gttggagaac aacattctga gggtaggagt cgaatattaa
     5761 gtaatcctga ctcaattagc cactgttttg aatccacata ctccaatact cctgaaatag
     5821 ttcattatgg acagcgcaga agagctgggg agaattaatt cgtaatcatg gtcatagctg
     5881 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     5941 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     6001 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     6061 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     6121 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     6181 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     6241 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     6301 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     6361 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     6421 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     6481 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     6541 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     6601 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     6661 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     6721 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     6781 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     6841 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     6901 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     6961 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     7021 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     7081 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     7141 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     7201 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     7261 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     7321 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     7381 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     7441 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     7501 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     7561 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     7621 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     7681 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     7741 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     7801 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     7861 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     7921 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     7981 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     8041 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     8101 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     8161 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     8221 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     8281 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     8341 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     8401 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     8461 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8521 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     8581 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     8641 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
     8701 tagtataata cgacaaggtg aggaaccata tgcggtgtga aataccgcac agatgcgtaa
     8761 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
     8821 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     8881 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     8941 ccaagctcgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
     9001 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
     9061 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
     9121 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     9181 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgattagt ccaatttgtt
     9241 aaagacagga tatcagtggt ccaggctcta gttttgactc aacaatatca ccagctgaag
     9301 cctatagagt acgagccata
//
