LOCUS       sequence-142267-        9414 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral expression plasmid for human NFATc1/bC .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9414)
  AUTHORS   Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, 
            Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel 
            H, Radbruch A, Krause E, Baumgrass R
  TITLE     Identification of Novel Nuclear Factor of Activated T Cells 
            (NFAT)-Associated Proteins in T cells.
  JOURNAL   J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
  PUBMED    27637333
REFERENCE   2  (bases 1 to 9414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Sep 16. pii: jbc.M116.739326."
COMMENT     Sequence Label: pMIG-hNFATc1/bC
FEATURES             Location/Qualifiers
     source          1..9414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             43..560
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    624..965
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1032..1448
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1378..1400
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1416..1432
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     4307..4323
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4308..4324
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4343..4909
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4359..4911
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4503..4520)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4730..4749
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4923..4925,4926..4928,4929..5642)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4923..4925,4926..4928,4929..5639)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4968..4989)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5229..5248)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5576..5597
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5733..6248
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(6417..6433)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6417..6433)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6430..6452)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6441..6457
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6465..6471,6472..6489,6490..6495))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6510..6531
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6648..6665)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6819..7407)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6899..6918)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7578..8369,8370..8438))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8201..8220
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8439..8543)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8611..8629
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8667..8689)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8789..8808
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9002..9024
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9016..9033
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9017..9033
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9236..9255
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg
       61 taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa tacataactg
      121 agaatagaga agttcagatc aaggttagga acagagagac agcagaatat gggccaaaca
      181 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga
      241 tgcggtcccg ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg
      301 acctgaaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
      361 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc
      421 agtcctccga tagactgcgt cgcccgggta cccgtattcc caataaagcc tcttgctgtt
      481 tgcatccgaa tcgtggactc gctgatcctt gggagggtct cctcagattg attgactgcc
      541 cacctcgggg gtctttcatt tggaggttcc accgagattt ggagacccct gcctagggac
      601 caccgacccc cccgccggga ggtaagctgg ccagcggtcg tttcgtgtct gtctctgtct
      661 ttgtgcgtgt ttgtgccggc atctaatgtt tgcgcctgcg tctgtactag ttagctaact
      721 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc
      781 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt
      841 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac
      901 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc
      961 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt
     1021 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag
     1081 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct
     1141 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc
     1201 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
     1261 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
     1321 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
     1381 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
     1441 taggcgccgg aattagatct gcggccgcgg atttaaatcc caccatgacg gggctggagg
     1501 accaggagtt cgacttcgag ttcctcttcg agtttaacca gcgcgacgag ggcgccgccg
     1561 cggccgcccc agaacactat ggctatgcat cctccaacgt cagccccgcc ctgccgctcc
     1621 ccacggcgca ctccaccctg ccggccccgt gccacaacct tcagacctcc acaccgggca
     1681 tcatcccgcc ggcggatcac ccctcggggt acggagcagc tttggacggt gggcccgcgg
     1741 gctacttcct ctcctccggc cacaccaggc ctgatggggc ccctgccctg gagagtcctc
     1801 gcatcgagat aacctcgtgc ttgggcctgt accacaacaa taaccagttt ttccacgatg
     1861 tggaggtgga agacgtcctc cctagctcca aacggtcccc ctccacggcc acgctgagtc
     1921 tgcccagcct ggaggcctac agagacccct cgtgcctgag cccggccagc agcctgtcct
     1981 cccggagctg caactcagag gcctcctcct acgagtccaa ctactcgtac ccgtacgcgt
     2041 ccccccagac gtcgccatgg cagtctccct gcgtgtctcc caagaccacg gaccccgagg
     2101 agggctttcc ccgcgggctg ggggcctgca cactgctggg ttccccgcgg cactccccct
     2161 ccacctcgcc ccgcgccagc gtcactgagg agagctggct gggtgcccgc tcctccagac
     2221 ccgcgtcccc ttgcaacaag aggaagtaca gcctcaacgg ccggcagccg ccctactcac
     2281 cccaccactc gcccacgccg tccccgcacg gctccccgcg ggtcagcgtg accgacgact
     2341 cgtggttggg caacaccacc cagtacacca gctcggccat cgtggccgcc atcaacgcgc
     2401 tgaccaccga cagcagcctg gacctgggag atggcgtccc tgtcaagtcc cgcaagacca
     2461 ccctggagca gccgccctca gtggcgctca aggtggagcc cgtcggggag gacctgggca
     2521 gccccccgcc cccggccgac ttcgcgcccg aagactactc ctctttccag cacatcagga
     2581 agggcggctt ctgcgaccag tacctggcgg tgccgcagca cccctaccag tgggcgaagc
     2641 ccaagcccct gtcccctacg tcctacatga gcccgaccct gcccgccctg gactggcagc
     2701 tgccgtccca ctcaggcccg tatgagcttc ggattgaggt gcagcccaag tcccaccacc
     2761 gagcccacta cgagacggag ggcagccggg gggccgtgaa ggcgtcggcc ggaggacacc
     2821 ccatcgtgca gctgcatggc tacttggaga atgagccgct gatgctgcag cttttcattg
     2881 ggacggcgga cgaccgcctg ctgcgcccgc acgccttcta ccaggtgcac cgcatcacag
     2941 ggaagaccgt gtccaccacc agccacgagg ccatcctctc caacaccaaa gtcctggaga
     3001 tcccactcct gccggagaac agcatgcgag ccgtcattga ctgtgccgga atcctgaaac
     3061 tcagaaactc cgacattgaa cttcggaaag gagagacgga catcgggagg aagaacacac
     3121 gggtacggct ggtgttccgc gttcacgtcc cgcaacccag cggccgcacg ctgtccctgc
     3181 aggtggcctc caaccccatc gaatgctccc agcgctcagc tcaggagctg cctctggtgg
     3241 agaagcagag cacggacagc tatccggtcg tgggcgggaa gaagatggtc ctgtctggcc
     3301 acaacttcct gcaggactcc aaggtcattt tcgtggagaa agccccagat ggccaccatg
     3361 tctgggagat ggaagcgaaa actgaccggg acctgtgcaa gccgaattct ctggtggttg
     3421 agatcccgcc atttcggaat cagaggataa ccagccccgt tcacgtcagt ttctacgtct
     3481 gcaacgggaa gagaaagcga agccagtacc agcgtttcac ctaccttccc gccaacgttc
     3541 caattataaa aacagaaccc actgatgatt atgagcctgc tccaacctgt ggaccggtga
     3601 gccaggggtt aagtcctctc ccaagaccat actacagcca gcagctcgcg atgccacccg
     3661 accccagctc ctgcctcgtg gccggcttcc cgccctgtcc gcagagaagc accctgatgc
     3721 cagcggcccc tggcgtgagc cccaagctcc acgacctttc tcccgctgcc tacaccaagg
     3781 gcgttgccag cccgggccac tgtcacctcg gactcccgca gccggccgga gaggcccccg
     3841 ccgtccagga cgtgcccagg ccagtggcca cgcaccccgg ctcgcccggg cagccacccc
     3901 cggccctgct gccacagcag gtgagtgcgc ctccaagcag tagctgcccc cctggtctcg
     3961 aacactcgct ctgccccagc agcccctctc ctccactccc gcctgccacc caagagccga
     4021 cctgcctgca gccctgcagc ccagcgtgcc cgcccgccac gggccgcccg cagcacctgc
     4081 cgtccacggt ccgcagggac gagtctccga ctgccgggcc acggctgctg ccagaggtgc
     4141 atgaggacgg tagtcctaat ttggccccta ttcctgtaac ggtcaagcga gagcctgaag
     4201 agttggacca gttgtacctg gatgacgtaa atgaaataat acgaaatgac ctctccagca
     4261 cgagcaccca ctcctaggcg atcgcttata agcggccgcg tcgaggtcga ggtcgacggt
     4321 atcgataagc ttgatatcga attccgcccc cccccctaac gttactggcc gaagccgctt
     4381 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     4441 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     4501 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     4561 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     4621 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     4681 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     4741 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     4801 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     4861 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa
     4921 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     4981 acggcgacgt aaacggccac aagttcagcg tgtctggcga gggcgagggc gatgccacct
     5041 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     5101 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     5161 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     5221 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     5281 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     5341 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     5401 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     5461 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     5521 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     5581 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     5641 aagaattcct gcagcccggg ggatccgtcg acctgcagcc aagcttatcg ataaaataaa
     5701 agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca
     5761 agctagctta attaacgcca ttttgcaagg catggaaaat acataactga gaatagagaa
     5821 gttcagatca aggttaggaa cagagagaca gcagaatatg ggccaaacag gatatctgtg
     5881 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtcccgc
     5941 cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaaatg
     6001 accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc
     6061 tgctccccga gctcaataaa agagcccaca acccctcact cggcgcgcca gtcctccgat
     6121 agactgcgtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt
     6181 gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg
     6241 tctttcatgg gtaacagttt cttgaagttg gagaacaaca ttctgagggt aggagtcgaa
     6301 tattaagtaa tcctgactca attagccact gttttgaatc cacatactcc aatactcctg
     6361 aaatagttca ttatggacag cgcagaagag ctggggagaa ttaattcgta atcatggtca
     6421 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     6481 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6541 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     6601 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     6661 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6721 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6781 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6841 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6901 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6961 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7021 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7081 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7141 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7201 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     7261 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7321 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7381 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7441 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7501 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7561 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7621 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7681 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7741 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7801 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7861 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7921 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7981 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     8041 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     8101 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     8161 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     8221 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     8281 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     8341 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     8401 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     8461 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     8521 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     8581 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     8641 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     8701 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     8761 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     8821 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
     8881 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     8941 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     9001 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tcgctctccc ttatgcgact
     9061 cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga
     9121 atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac
     9181 ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga
     9241 tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc
     9301 gtccggcgta gaggatcgat tagtccaatt tgttaaagac aggatatcag tggtccaggc
     9361 tctagttttg actcaacaat atcaccagct gaagcctata gagtacgagc cata
//
