LOCUS       sequence-142271-        9224 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral expression plasmid for human NFATc2/C (without C-terminal
            region) with C-terminal BirA-biotinylation signal and TEV celavage 
            site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9224)
  AUTHORS   Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, 
            Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel 
            H, Radbruch A, Krause E, Baumgrass R
  TITLE     Identification of Novel Nuclear Factor of Activated T Cells 
            (NFAT)-Associated Proteins in T cells.
  JOURNAL   J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
  PUBMED    27637333
REFERENCE   2  (bases 1 to 9224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Sep 16. pii: jbc.M116.739326."
COMMENT     Sequence Label: pMIG-hNFATc2/C(1-686)AVITEV
FEATURES             Location/Qualifiers
     source          1..9224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             43..560
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    624..965
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1032..1448
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1378..1400
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1416..1432
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             3549..3569
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3582..3626
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     primer_bind     3665..3681
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3666..3682
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3701..4267
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3717..4269
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3861..3878)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4088..4107
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4281..4283,4284..4286,4287..5000)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4281..4283,4284..4286,4287..4997)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4326..4347)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4587..4606)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4934..4955
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5091..5606
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(5775..5791)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5775..5791)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5788..5810)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5799..5815
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5823..5829,5830..5847,5848..5853))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5868..5889
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6006..6023)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6177..6765)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6257..6276)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6936..7727,7728..7796))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7559..7578
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7797..7901)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7969..7987
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8025..8047)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8147..8166
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8189..8209)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8194..8523
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8374..8509
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8436..8455
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8571..8618
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     8812..8834
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8826..8843
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8827..8843
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9046..9065
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg
       61 taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa tacataactg
      121 agaatagaga agttcagatc aaggttagga acagagagac agcagaatat gggccaaaca
      181 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga
      241 tgcggtcccg ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg
      301 acctgaaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
      361 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc
      421 agtcctccga tagactgcgt cgcccgggta cccgtattcc caataaagcc tcttgctgtt
      481 tgcatccgaa tcgtggactc gctgatcctt gggagggtct cctcagattg attgactgcc
      541 cacctcgggg gtctttcatt tggaggttcc accgagattt ggagacccct gcctagggac
      601 caccgacccc cccgccggga ggtaagctgg ccagcggtcg tttcgtgtct gtctctgtct
      661 ttgtgcgtgt ttgtgccggc atctaatgtt tgcgcctgcg tctgtactag ttagctaact
      721 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc
      781 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt
      841 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac
      901 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc
      961 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt
     1021 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag
     1081 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct
     1141 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc
     1201 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
     1261 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
     1321 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
     1381 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
     1441 taggcgccgg aattagatct gcggccgcgg atttaaatcc caccatgaac gcccccgagc
     1501 ggcagcccca acccgacggc ggggacgccc caggccacga gcctgggggc agcccccaag
     1561 acgagcttga cttctccatc ctcttcgact atgagtattt gaatccgaac gaagaagagc
     1621 cgaatgcaca taaggtcgcc agcccaccct ccggacccgc ataccccgat gatgtcctgg
     1681 actatggcct caagccatac agcccccttg ctagtctctc tggcgagccc cccggccgat
     1741 tcggagagcc ggatagggta gggccgcaga agtttctgag cgcggccaag ccagcagggg
     1801 cctcgggcct gagccctcgg atcgagatca ctccgtccca cgaactgatc caggcagtgg
     1861 ggcccctccg catgagagac gcgggcctcc tggtggagca gccgcccctg gccggggtgg
     1921 ccgccagccc gaggttcacc ctgcccgtgc ccggcttcga gggctaccgc gagccgcttt
     1981 gcttgagccc cgctagcagc ggctcctctg ccagcttcat ttctgacacc ttctccccct
     2041 acacctcgcc ctgcgtctcg cccaataacg gcgggcccga cgacctgtgt ccgcagtttc
     2101 aaaacatccc tgctcattat tcccccagaa cctcgccaat aatgtcacct cgaaccagcc
     2161 tcgccgagga cagctgcctg ggccgccact cgcccgtgcc ccgtccggcc tcccgctcct
     2221 catcgcctgg tgccaagcgg aggcattcgt gcgccgaggc cttggttgcc ctgccgcccg
     2281 gagcctcacc ccagcgctcc cggagcccct cgccgcagcc ctcatctcac gtggcacccc
     2341 aggaccacgg ctccccggct gggtaccccc ctgtggctgg ctctgccgtg atcatggatg
     2401 ccctgaacag cctcgccacg gactcgcctt gtgggatccc ccccaagatg tggaagacca
     2461 gccctgaccc ctcgccggtg tctgccgccc catccaaggc cggcctgcct cgccacatct
     2521 acccggccgt ggagttcctg gggccctgcg agcagggcga gaggagaaac tcggctccag
     2581 aatccatcct gctggttccg cccacttggc ccaagccgct ggtgcctgcc attcccatct
     2641 gcagcatccc agtgactgca tccctccctc cacttgagtg gccgctgtcc agtcagtcag
     2701 gctcttacga gctgcggatc gaggtgcagc ccaagccaca tcaccgggcc cactatgaga
     2761 cagaaggcag ccgaggggct gtcaaagctc caactggagg ccaccctgtg gttcagctcc
     2821 atggctacat ggaaaacaag cctctgggac ttcagatctt cattgggaca gctgatgagc
     2881 ggatccttaa gccgcacgcc ttctaccagg tgcaccgaat cacggggaaa actgtcacca
     2941 ccaccagcta tgagaagata gtgggcaaca ccaaagtcct ggagatcccc ttggagccca
     3001 aaaacaacat gagggcaacc atcgactgtg cggggatctt gaagcttaga aacgccgaca
     3061 ttgagctgcg gaaaggcgag acggacattg gaagaaagaa cacgcgggtg agactggttt
     3121 tccgagttca catcccagag tccagtggca gaatcgtctc tttacagact gcatctaacc
     3181 ccatcgagtg ctcccagcga tctgctcacg agctgcccat ggttgaaaga caagacacag
     3241 acagctgcct ggtctatggc ggccagcaaa tgatcctcac ggggcagaac tttacatccg
     3301 agtccaaagt tgtgtttact gagaagacca cagatggaca gcaaatttgg gagatggaag
     3361 ccacggtgga taaggacaag agccagccca acatgctttt tgttgagatc cctgaatatc
     3421 ggaacaagca tatccgcaca cctgtaaaag tgaacttcta cgtcatcaat gggaagagaa
     3481 aacgaagtca gcctcagcac tttacctacc acccagtccc agccatcaag acggagccca
     3541 cgtacgtaga gaacctgtac ttccagggcg ttaacggcgg tggcctgaac gatatcttcg
     3601 aggcacagaa aatcgaatgg catgaggtta actgagcgat cgcttataag cggccgcgtc
     3661 gaggtcgagg tcgacggtat cgataagctt gatatcgaat tccgcccccc cccctaacgt
     3721 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     3781 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     3841 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     3901 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     3961 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     4021 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     4081 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     4141 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     4201 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     4261 atgataatat ggccacaacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     4321 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tctggcgagg
     4381 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     4441 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     4501 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     4561 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     4621 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     4681 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     4741 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     4801 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     4861 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     4921 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     4981 tggacgagct gtacaagtaa gaattcctgc agcccggggg atccgtcgac ctgcagccaa
     5041 gcttatcgat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc
     5101 ccacctgtag gtttggcaag ctagcttaat taacgccatt ttgcaaggca tggaaaatac
     5161 ataactgaga atagagaagt tcagatcaag gttaggaaca gagagacagc agaatatggg
     5221 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt
     5281 ccccagatgc ggtcccgccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc
     5341 cccaaggacc tgaaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg
     5401 cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg
     5461 gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtgtatccaa taaaccctct
     5521 tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg
     5581 actacccgtc agcgggggtc tttcatgggt aacagtttct tgaagttgga gaacaacatt
     5641 ctgagggtag gagtcgaata ttaagtaatc ctgactcaat tagccactgt tttgaatcca
     5701 catactccaa tactcctgaa atagttcatt atggacagcg cagaagagct ggggagaatt
     5761 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     5821 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     5881 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     5941 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     6001 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6061 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6121 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6181 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6241 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6301 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6361 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     6421 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     6481 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     6541 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     6601 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     6661 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     6721 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     6781 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     6841 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     6901 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     6961 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7021 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7081 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7141 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7201 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     7261 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     7321 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     7381 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     7441 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     7501 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     7561 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     7621 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     7681 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     7741 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     7801 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     7861 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     7921 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     7981 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     8041 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     8101 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     8161 attgtactga gagtgcacca tatgctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     8221 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     8281 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     8341 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     8401 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     8461 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     8521 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt
     8581 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac catatgcggt
     8641 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
     8701 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     8761 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     8821 gacgttgtaa aacgacggcc agtgccaagc tcgctctccc ttatgcgact cctgcattag
     8881 gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg
     8941 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa
     9001 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat
     9061 ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta
     9121 gaggatcgat tagtccaatt tgttaaagac aggatatcag tggtccaggc tctagttttg
     9181 actcaacaat atcaccagct gaagcctata gagtacgagc cata
//
