LOCUS       sequence-142272-        9254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral expression plasmid for human NFATc2/C(1-686, RIT) 
            (without C-terminal region, with RIT mutation abrogating AP1 
            binding) with C-terminal BirA-biotinylation signal and TEV celavage 
            site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9254)
  AUTHORS   Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, 
            Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel 
            H, Radbruch A, Krause E, Baumgrass R
  TITLE     Identification of Novel Nuclear Factor of Activated T Cells 
            (NFAT)-Associated Proteins in T cells.
  JOURNAL   J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
  PUBMED    27637333
REFERENCE   2  (bases 1 to 9254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Sep 16. pii: jbc.M116.739326."
COMMENT     Sequence Label: pMIG-hNFATc2/C(1-686, RIT)FLAG-AVITEV
FEATURES             Location/Qualifiers
     source          1..9254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             43..560
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    624..965
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1032..1448
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1378..1400
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1416..1432
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             3549..3572
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3579..3599
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3612..3656
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     primer_bind     3695..3711
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3696..3712
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3731..4297
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3747..4299
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3891..3908)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4118..4137
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4311..4313,4314..4316,4317..5030)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4311..4313,4314..4316,4317..5027)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4356..4377)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4617..4636)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4964..4985
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5121..5636
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(5805..5821)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5805..5821)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5818..5840)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5829..5845
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5853..5859,5860..5877,5878..5883))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5898..5919
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6036..6053)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6207..6795)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6287..6306)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6966..7757,7758..7826))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7589..7608
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7827..7931)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7999..8017
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8055..8077)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8177..8196
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8219..8239)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8224..8553
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8404..8539
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8466..8485
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8601..8648
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     8842..8864
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8856..8873
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8857..8873
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9076..9095
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg
       61 taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa tacataactg
      121 agaatagaga agttcagatc aaggttagga acagagagac agcagaatat gggccaaaca
      181 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga
      241 tgcggtcccg ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg
      301 acctgaaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
      361 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc
      421 agtcctccga tagactgcgt cgcccgggta cccgtattcc caataaagcc tcttgctgtt
      481 tgcatccgaa tcgtggactc gctgatcctt gggagggtct cctcagattg attgactgcc
      541 cacctcgggg gtctttcatt tggaggttcc accgagattt ggagacccct gcctagggac
      601 caccgacccc cccgccggga ggtaagctgg ccagcggtcg tttcgtgtct gtctctgtct
      661 ttgtgcgtgt ttgtgccggc atctaatgtt tgcgcctgcg tctgtactag ttagctaact
      721 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc
      781 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt
      841 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac
      901 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc
      961 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt
     1021 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag
     1081 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct
     1141 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc
     1201 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
     1261 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
     1321 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
     1381 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
     1441 taggcgccgg aattagatct gcggccgcgg atttaaatcc caccatgaac gcccccgagc
     1501 ggcagcccca acccgacggc ggggacgccc caggccacga gcctgggggc agcccccaag
     1561 acgagcttga cttctccatc ctcttcgact atgagtattt gaatccgaac gaagaagagc
     1621 cgaatgcaca taaggtcgcc agcccaccct ccggacccgc ataccccgat gatgtcctgg
     1681 actatggcct caagccatac agcccccttg ctagtctctc tggcgagccc cccggccgat
     1741 tcggagagcc ggatagggta gggccgcaga agtttctgag cgcggccaag ccagcagggg
     1801 cctcgggcct gagccctcgg atcgagatca ctccgtccca cgaactgatc caggcagtgg
     1861 ggcccctccg catgagagac gcgggcctcc tggtggagca gccgcccctg gccggggtgg
     1921 ccgccagccc gaggttcacc ctgcccgtgc ccggcttcga gggctaccgc gagccgcttt
     1981 gcttgagccc cgctagcagc ggctcctctg ccagcttcat ttctgacacc ttctccccct
     2041 acacctcgcc ctgcgtctcg cccaataacg gcgggcccga cgacctgtgt ccgcagtttc
     2101 aaaacatccc tgctcattat tcccccagaa cctcgccaat aatgtcacct cgaaccagcc
     2161 tcgccgagga cagctgcctg ggccgccact cgcccgtgcc ccgtccggcc tcccgctcct
     2221 catcgcctgg tgccaagcgg aggcattcgt gcgccgaggc cttggttgcc ctgccgcccg
     2281 gagcctcacc ccagcgctcc cggagcccct cgccgcagcc ctcatctcac gtggcacccc
     2341 aggaccacgg ctccccggct gggtaccccc ctgtggctgg ctctgccgtg atcatggatg
     2401 ccctgaacag cctcgccacg gactcgcctt gtgggatccc ccccaagatg tggaagacca
     2461 gccctgaccc ctcgccggtg tctgccgccc catccaaggc cggcctgcct cgccacatct
     2521 acccggccgt ggagttcctg gggccctgcg agcagggcga gaggagaaac tcggctccag
     2581 aatccatcct gctggttccg cccacttggc ccaagccgct ggtgcctgcc attcccatct
     2641 gcagcatccc agtgactgca tccctccctc cacttgagtg gccgctgtcc agtcagtcag
     2701 gctcttacga gctgcggatc gaggtgcagc ccaagccaca tcaccgggcc cactatgaga
     2761 cagaaggcag ccgaggggct gtcaaagctc caactggagg ccaccctgtg gttcagctcc
     2821 atggctacat ggaaaacaag cctctgggac ttcagatctt cattgggaca gctgatgagg
     2881 ccgcccttaa gccgcacgcc ttctaccagg tgcaccgaat cacggggaaa actgtcacca
     2941 ccaccagcta tgagaagata gtgggcaaca ccaaagtcct ggagatcccc ttggagccca
     3001 aaaacaacat gagggcaacc atcgactgtg cggggatctt gaagcttaga aacgccgaca
     3061 ttgagctgcg gaaaggcgag gccgacattg gaagaaagaa cacgcgggtg agactggttt
     3121 tccgagttca catcccagag tccagtggca gaatcgtctc tttacagact gcatctaacc
     3181 ccatcgagtg ctcccagcga tctgctcacg agctgcccat ggttgaaaga caagacacag
     3241 acagctgcct ggtctatggc ggccagcaaa tgatcctcac ggggcagaac tttacatccg
     3301 agtccaaagt tgtgtttact gagaagacca cagatggaca gcaaatttgg gagatggaag
     3361 ccacggtgga taaggacaag agccagccca acatgctttt tgttgagatc cctgaatatc
     3421 ggaacaagca tatccgcaca cctgtaaaag tgaacttcta cgtcatcaat gggaagagaa
     3481 aacgaagtca gcctcagcac tttacctacc acccagtccc agccatcaag acggagccca
     3541 cgtacgtaga ttacaaggat gacgacgata agcttgtaga gaacctgtac ttccagggcg
     3601 ttaacggcgg tggcctgaac gatatcttcg aggcacagaa aatcgaatgg catgaggtta
     3661 actgagcgat cgcttataag cggccgcgtc gaggtcgagg tcgacggtat cgataagctt
     3721 gatatcgaat tccgcccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     3781 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     3841 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     3901 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     3961 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     4021 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     4081 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     4141 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     4201 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     4261 cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacaacc atggtgagca
     4321 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     4381 acggccacaa gttcagcgtg tctggcgagg gcgagggcga tgccacctac ggcaagctga
     4441 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     4501 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     4561 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     4621 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     4681 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     4741 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     4801 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     4861 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     4921 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     4981 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa gaattcctgc
     5041 agcccggggg atccgtcgac ctgcagccaa gcttatcgat aaaataaaag attttattta
     5101 gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaat
     5161 taacgccatt ttgcaaggca tggaaaatac ataactgaga atagagaagt tcagatcaag
     5221 gttaggaaca gagagacagc agaatatggg ccaaacagga tatctgtggt aagcagttcc
     5281 tgccccggct cagggccaag aacagatggt ccccagatgc ggtcccgccc tcagcagttt
     5341 ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaaatgac cctgtgcctt
     5401 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
     5461 tcaataaaag agcccacaac ccctcactcg gcgcgccagt cctccgatag actgcgtcgc
     5521 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg
     5581 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatgggt
     5641 aacagtttct tgaagttgga gaacaacatt ctgagggtag gagtcgaata ttaagtaatc
     5701 ctgactcaat tagccactgt tttgaatcca catactccaa tactcctgaa atagttcatt
     5761 atggacagcg cagaagagct ggggagaatt aattcgtaat catggtcata gctgtttcct
     5821 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     5881 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     5941 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     6001 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     6061 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     6121 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6181 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6241 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6301 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6361 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     6421 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6481 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6541 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6601 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     6661 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6721 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6781 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6841 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     6901 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     6961 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7021 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7081 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7141 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7201 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7261 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7321 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7381 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     7441 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     7501 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     7561 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7621 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7681 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7741 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7801 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     7861 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     7921 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     7981 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     8041 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     8101 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     8161 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgctgtgg
     8221 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     8281 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     8341 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     8401 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     8461 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     8521 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     8581 ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat
     8641 aatacgacaa ggtgaggaac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     8701 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     8761 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     8821 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc
     8881 tcgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt
     8941 gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc
     9001 cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc
     9061 ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc
     9121 ggtgatgccg gccacgatgc gtccggcgta gaggatcgat tagtccaatt tgttaaagac
     9181 aggatatcag tggtccaggc tctagttttg actcaacaat atcaccagct gaagcctata
     9241 gagtacgagc cata
//
