LOCUS       sequence-142273-        9951 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral expression plasmid for human NFATc2/C (with RIT mutation 
            abrogating AP1 binding) with C-terminal BirA-biotinylation signal 
            and TEV celavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9951)
  AUTHORS   Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, 
            Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel 
            H, Radbruch A, Krause E, Baumgrass R
  TITLE     Identification of Novel Nuclear Factor of Activated T Cells 
            (NFAT)-Associated Proteins in T cells.
  JOURNAL   J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
  PUBMED    27637333
REFERENCE   2  (bases 1 to 9951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Sep 16. pii: jbc.M116.739326."
COMMENT     Sequence Label: pMIG-hNFATc2/C(RIT)-FLAG-AVITEV
FEATURES             Location/Qualifiers
     source          1..9951
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             43..560
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    624..965
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1032..1448
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1378..1400
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1416..1432
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      3631..3640
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4246..4269
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4276..4296
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4309..4353
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     primer_bind     4392..4408
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4393..4409
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4428..4994
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4444..4996
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4588..4605)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4815..4834
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5008..5010,5011..5013,5014..5727)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5008..5010,5011..5013,5014..5724)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5053..5074)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5314..5333)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5661..5682
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5818..6333
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(6502..6518)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6502..6518)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6515..6537)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6526..6542
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6550..6556,6557..6574,6575..6580))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6595..6616
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6733..6750)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6904..7492)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6984..7003)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7663..8454,8455..8523))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8286..8305
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8524..8628)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8696..8714
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8752..8774)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8874..8893
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8916..8936)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8921..9250
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9101..9236
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9163..9182
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9298..9345
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     9539..9561
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9553..9570
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9554..9570
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9773..9792
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg
       61 taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa tacataactg
      121 agaatagaga agttcagatc aaggttagga acagagagac agcagaatat gggccaaaca
      181 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga
      241 tgcggtcccg ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg
      301 acctgaaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
      361 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc
      421 agtcctccga tagactgcgt cgcccgggta cccgtattcc caataaagcc tcttgctgtt
      481 tgcatccgaa tcgtggactc gctgatcctt gggagggtct cctcagattg attgactgcc
      541 cacctcgggg gtctttcatt tggaggttcc accgagattt ggagacccct gcctagggac
      601 caccgacccc cccgccggga ggtaagctgg ccagcggtcg tttcgtgtct gtctctgtct
      661 ttgtgcgtgt ttgtgccggc atctaatgtt tgcgcctgcg tctgtactag ttagctaact
      721 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc
      781 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt
      841 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac
      901 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc
      961 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt
     1021 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag
     1081 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct
     1141 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc
     1201 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
     1261 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
     1321 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
     1381 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
     1441 taggcgccgg atttaaatcc caccatgaac gcccccgagc ggcagcccca acccgacggc
     1501 ggggacgccc caggccacga gcctgggggc agcccccaag acgagcttga cttctccatc
     1561 ctcttcgact atgagtattt gaatccgaac gaagaagagc cgaatgcaca taaggtcgcc
     1621 agcccaccct ccggacccgc ataccccgat gatgtcctgg actatggcct caagccatac
     1681 agcccccttg ctagtctctc tggcgagccc cccggccgat tcggagagcc ggatagggta
     1741 gggccgcaga agtttctgag cgcggccaag ccagcagggg cctcgggcct gagccctcgg
     1801 atcgagatca ctccgtccca cgaactgatc caggcagtgg ggcccctccg catgagagac
     1861 gcgggcctcc tggtggagca gccgcccctg gccggggtgg ccgccagccc gaggttcacc
     1921 ctgcccgtgc ccggcttcga gggctaccgc gagccgcttt gcttgagccc cgctagcagc
     1981 ggctcctctg ccagcttcat ttctgacacc ttctccccct acacctcgcc ctgcgtctcg
     2041 cccaataacg gcgggcccga cgacctgtgt ccgcagtttc aaaacatccc tgctcattat
     2101 tcccccagaa cctcgccaat aatgtcacct cgaaccagcc tcgccgagga cagctgcctg
     2161 ggccgccact cgcccgtgcc ccgtccggcc tcccgctcct catcgcctgg tgccaagcgg
     2221 aggcattcgt gcgccgaggc cttggttgcc ctgccgcccg gagcctcacc ccagcgctcc
     2281 cggagcccct cgccgcagcc ctcatctcac gtggcacccc aggaccacgg ctccccggct
     2341 gggtaccccc ctgtggctgg ctctgccgtg atcatggatg ccctgaacag cctcgccacg
     2401 gactcgcctt gtgggatccc ccccaagatg tggaagacca gccctgaccc ctcgccggtg
     2461 tctgccgccc catccaaggc cggcctgcct cgccacatct acccggccgt ggagttcctg
     2521 gggccctgcg agcagggcga gaggagaaac tcggctccag aatccatcct gctggttccg
     2581 cccacttggc ccaagccgct ggtgcctgcc attcccatct gcagcatccc agtgactgca
     2641 tccctccctc cacttgagtg gccgctgtcc agtcagtcag gctcttacga gctgcggatc
     2701 gaggtgcagc ccaagccaca tcaccgggcc cactatgaga cagaaggcag ccgaggggct
     2761 gtcaaagctc caactggagg ccaccctgtg gttcagctcc atggctacat ggaaaacaag
     2821 cctctgggac ttcagatctt cattgggaca gctgatgagg ccgcccttaa gccgcacgcc
     2881 ttctaccagg tgcaccgaat cacggggaaa actgtcacca ccaccagcta tgagaagata
     2941 gtgggcaaca ccaaagtcct ggagatcccc ttggagccca aaaacaacat gagggcaacc
     3001 atcgactgtg cggggatctt gaagcttaga aacgccgaca ttgagctgcg gaaaggcgag
     3061 gccgacattg gaagaaagaa cacgcgggtg agactggttt tccgagttca catcccagag
     3121 tccagtggca gaatcgtctc tttacagact gcatctaacc ccatcgagtg ctcccagcga
     3181 tctgctcacg agctgcccat ggttgaaaga caagacacag acagctgcct ggtctatggc
     3241 ggccagcaaa tgatcctcac ggggcagaac tttacatccg agtccaaagt tgtgtttact
     3301 gagaagacca cagatggaca gcaaatttgg gagatggaag ccacggtgga taaggacaag
     3361 agccagccca acatgctttt tgttgagatc cctgaatatc ggaacaagca tatccgcaca
     3421 cctgtaaaag tgaacttcta cgtcatcaat gggaagagaa aacgaagtca gcctcagcac
     3481 tttacctacc acccagtccc agccatcaag acggagccca cggatgaata tgaccccact
     3541 ctgatctgca gccccaccca tggaggcctg gggagccagc cttactaccc ccagcacccg
     3601 atggtggccg agtccccctc ctgcctcgtg gccaccatgg ctccctgcca gcagttccgc
     3661 acggggctct catcccctga cgcccgctac cagcaacaga acccagcggc cgtactctac
     3721 cagcggagca agagcctgag ccccagcctg ctgggctatc agcagccggc cctcatggcc
     3781 gccccgctgt cccttgcgga cgctcaccgc tctgtgctgg tgcacgccgg ctcccagggc
     3841 cagagctcag ccctgctcca cccctctccg accaaccagc aggcctcgcc tgtgatccac
     3901 tactcaccca ccaaccagca gctgcgctgc ggaagccacc aggagttcca gcacatcatg
     3961 tactgcgaga atttcgcacc aggcaccacc agacctggcc cgcccccggt cagtcaaggt
     4021 cagaggctga gcccgggttc ctaccccaca gtcattcagc agcagaatgc cacgagccaa
     4081 agagccgcca aaaacggacc cccggtcagt gaccaaaagg aagtattacc tgcgggggtg
     4141 accattaaac aggagcagaa cttggaccag acctacttgg atgatgttaa tgaaattatc
     4201 aggaaggagt tttcaggacc tcctgccaga aatcagacgt acgtagatta caaggatgac
     4261 gacgataagc ttgtagagaa cctgtacttc cagggcgtta acggcggtgg cctgaacgat
     4321 atcttcgagg cacagaaaat cgaatggcat gaggttaact gagcgatcgc ttataagcgg
     4381 ccgcgtcgag gtcgaggtcg acggtatcga taagcttgat atcgaattcc gccccccccc
     4441 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
     4501 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     4561 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     4621 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     4681 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     4741 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     4801 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     4861 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
     4921 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     4981 aaacacgatg ataatatggc cacaaccatg gtgagcaagg gcgaggagct gttcaccggg
     5041 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtct
     5101 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     5161 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     5221 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     5281 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     5341 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     5401 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     5461 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     5521 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     5581 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     5641 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     5701 ctcggcatgg acgagctgta caagtaagaa ttcctgcagc ccgggggatc cgtcgacctg
     5761 cagccaagct tatcgataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg
     5821 aaagacccca cctgtaggtt tggcaagcta gcttaattaa cgccattttg caaggcatgg
     5881 aaaatacata actgagaata gagaagttca gatcaaggtt aggaacagag agacagcaga
     5941 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac
     6001 agatggtccc cagatgcggt cccgccctca gcagtttcta gagaaccatc agatgtttcc
     6061 agggtgcccc aaggacctga aaatgaccct gtgccttatt tgaactaacc aatcagttcg
     6121 cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc
     6181 tcactcggcg cgccagtcct ccgatagact gcgtcgcccg ggtacccgtg tatccaataa
     6241 accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga
     6301 gtgattgact acccgtcagc gggggtcttt catgggtaac agtttcttga agttggagaa
     6361 caacattctg agggtaggag tcgaatatta agtaatcctg actcaattag ccactgtttt
     6421 gaatccacat actccaatac tcctgaaata gttcattatg gacagcgcag aagagctggg
     6481 gagaattaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     6541 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     6601 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6661 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     6721 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     6781 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     6841 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6901 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     6961 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7021 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7081 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7141 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7201 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     7261 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7321 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     7381 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     7441 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7501 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     7561 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     7621 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     7681 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     7741 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     7801 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     7861 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     7921 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     7981 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     8041 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     8101 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     8161 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     8221 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     8281 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     8341 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     8401 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     8461 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     8521 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     8581 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     8641 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     8701 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     8761 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     8821 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     8881 agagcagatt gtactgagag tgcaccatat gctgtggaat gtgtgtcagt tagggtgtgg
     8941 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     9001 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     9061 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     9121 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     9181 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     9241 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt
     9301 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaaccat
     9361 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
     9421 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     9481 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     9541 cagtcacgac gttgtaaaac gacggccagt gccaagctcg ctctccctta tgcgactcct
     9601 gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg
     9661 gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca
     9721 cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt
     9781 cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc
     9841 cggcgtagag gatcgattag tccaatttgt taaagacagg atatcagtgg tccaggctct
     9901 agttttgact caacaatatc accagctgaa gcctatagag tacgagccat a
//
