LOCUS       sequence-142328-         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Third generation lentiviral vector expressing GFP and puromycin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Witwicka H, Hwang SY, Reyes-Gutierrez P, Jia H, Odgren PE, Donahue 
            LR, Birnbaum MJ, Odgren PR
  TITLE     Studies of OC-STAMP in Osteoclast Fusion: A New Knockout Mouse 
            Model, Rescue of Cell Fusion, and Transmembrane Topology.
  JOURNAL   PLoS One. 2015 Jun 4;10(6):e0128275. doi: 
            10.1371/journal.pone.0128275. eCollection 2015.
  PUBMED    26042409
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-06-4"; volume: "10(6):e0128275. doi"; pages: " 
            10.1371/journal.pone.0128275. eCollection 2015"
COMMENT     Sequence Label: pLenti-CMV-MCS-GFP-SV-puro
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(77..101)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(98..301)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(127..147)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        302..605
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    628..744
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcctcgccc ttgctcacca ttctagagtc ggtgtcttct atggaggtca aaacagcgtg
       61 gatggcgtct ccaggcgatc tgacggttca ctaaacgagc tctgcttata tagacctccc
      121 accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa
      181 agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg
      241 gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca
      301 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
      361 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
      421 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
      481 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
      541 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
      601 taacgcggaa ctcccaagct tatcgataaa attttgaatt ttgtaatttg tttttgtaat
      661 tctttagttt gtatgtctgt tgctattatg tctactattc tttcccctgc actgtacccc
      721 ccaatccccc cttttctttt aaaagttaac cgataccgtc gagatccgtt cactaatcga
      781 atggatctgt ctctgtctct ctctccacct tcttcttcta ttccttcggg cctgtcgggt
      841 cccctcgggg ttgggaggtg ggtctgaaac gataatggtg aatatccctg cctaactcta
      901 ttcactatag aaagtacagc aaaaactatt cttaaaccta cca
//
