LOCUS       sequence-142330-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; 
            kinase dead) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Shi H, Wang Y, Li X, Zhan X, Tang M, Fina M, Su L, Pratt D, Bu CH, 
            Hildebrand S, Lyon S, Scott L, Quan J, Sun Q, Russell J, Arnett S, 
            Jurek P, Chen D, Kravchenko VV, Mathison JC, Moresco EM, Monson NL, 
            Ulevitch RJ, Beutler B
  TITLE     NLRP3 activation and mitosis are mutually exclusive events 
            coordinated by NEK7, a new inflammasome component.
  JOURNAL   Nat Immunol. 2016 Mar;17(3):250-8. doi: 10.1038/ni.3333. Epub 2015 
            Dec 7.
  PUBMED    26642356
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.3333"; 
            journalName: "Nat Immunol"; date: "2016-03"; volume: "17"; issue: 
            "3"; pages: "250-8"
COMMENT     Sequence Label: pcDNA3-N-HA-NEK7 K64M
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             251..259
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atggatgaac aatcacaagg aatgcaaggg ccgcccgtta ctcagttcca gccacagaag
       61 gcattacggc cagatatggg ctataatact ttagccaact tccgaataga aaagaaaatt
      121 ggtcgtggac aatttagtga agtttataga gcatcctgtc tcttggatgg agtgccggta
      181 gcgttaaaaa tggtacagat atttgattta atggatgcca aagcacgtgc tgattgtatc
      241 aaagaaatag acctccttaa gcaactcaac catccaaatg taattaaata ctatgcatca
      301 ttcattgagg ataatgagct gaacatagtt ttggagttag cagatgctgg tgacctctcc
      361 agaatgataa agcactttaa gaaacaaaag aggctaatcc ctgagagaac cgtttggaaa
      421 tacttcgttc agctctgcag tgcactggac cacatgcatt ctcgaagagt catgcacaga
      481 gatataaaac cagctaatgt gttcattaca gccactgggg tagtaaaact cggagacctt
      541 gggcttggtc ggtttttcag ctccaaaacc acagctgcac attctttagt gggtacacct
      601 tactacatgt ctccagagag aatacatgaa aatggataca acttcaagtc tgacatctgg
      661 tctcttggct gtctgctata tgagatggct gcactgcaga gtcctttcta cggcgacaag
      721 atgaacttgt attctctgtg taagaagata gagcagtgtg actacccgcc tctcccgtca
      781 gatcactatt cggaggagct acgacagcta gttaatatat gcatcaaccc agatccagag
      841 aagcgacccg acatcgccta tgtttatgat gtggcaaaga ggatgcatgc atgtaccgca
      901 agcacctaa
//
