LOCUS       sequence-142333-         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FlAsH-BRET reporter to monitor conformational changes in Arrestin3. 
            Insertion of FlAsH motif (CCPGCC) following amino acid residue 40 of
            Arrestin3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Lee MH, Appleton KM, Strungs EG, Kwon JY, Morinelli TA, Peterson YK,
            Laporte SA, Luttrell LM
  TITLE     The conformational signature of beta-arrestin2 predicts its 
            trafficking and signalling functions.
  JOURNAL   Nature. 2016 Mar 31;531(7596):665-8. doi: 10.1038/nature17154. Epub 
            2016 Mar 23.
  PUBMED    27007854
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17154"; journalName: "Nature"; date: "2016-03-31- 
            31"; volume: "531"; issue: "7596"; pages: "665-8"
COMMENT     Sequence Label: Rlucrarr3-Flash1
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             198..215
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
ORIGIN
        1 acgagcaggg ggatctccgg cgagctctcg agaattctca cgcgtctgca ggatatcaag
       61 cttgcgttac cggatccatg ggagagaaac cagggaccag ggtcttcaag aagtcgagcc
      121 ctaactgcaa gctcaccgtg tacttgggca agcgtgactt tgtggatcac ttggacaaag
      181 tggatcctgt cgatggttgt tgtcctggtt gttgtgtggt gcttgtggat cctgactact
      241 tgaaggaccg gaaagtgttt gtgaccctca cctgtgcctt ccgctatggc cgagaagacc
      301 tggatgtact gggcctgtct ttccgcaaag atctgttcat cgccacctac caggccttcc
      361 cccccatgcc caacccacct cggcccccca cccgcctaca ggaccgactg ctgaagaagt
      421 tgggccagca tgcccacccc ttttttttca caatacccca gaatttgcct tgctccgtca
      481 cactgcagcc aggaccggag gacacaggga aggcctgtgg agtagacttt gagattcgag
      541 ccttctgtgc caaatctata gaagaaaaaa gccacaaaag gaactccgtg cggcttatca
      601 tcagaaaggt acagtttgct cctgagacac ccggccccca gccatcagct gaaaccacac
      661 gccacttcct catgtctgac cggaggtccc tgcacctaga ggcttccctg gacaaagagc
      721 tgtactacca tggggaaccc ctcaatgtca acgtccacgt caccaacaat tctgccaaga
      781 ccgtcaagaa gatcagagtg tctgtgagac agta
//
