LOCUS       sequence-142347-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This a retroviral expression plasmid expressing GFP along with hygro
            resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Agalioti T, Giannou AD, Krontira AC, Kanellakis NI, Kati D, Vreka M,
            Pepe M, Spella M, Lilis I, Zazara DE, Nikolouli E, Spiropoulou N, 
            Papadakis A, Papadia K, Voulgaridis A, Harokopos V, Stamou P, 
            Meiners S, Eickelberg O, Snyder LA, Antimisiaris SG, Kardamakis D, 
            Psallidas I, Marazioti A, Stathopoulos GT
  TITLE     Mutant KRAS promotes malignant pleural effusion formation.
  JOURNAL   Nat Commun. 2017 May 16;8:15205. doi: 10.1038/ncomms15205.
  PUBMED    28508873
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-05-16"; volume: "8:15205. doi"; pages: " 
            10.1038/ncomms15205"
COMMENT     Sequence Label: 8200 Bicistronic_GFP_ires_hygro
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tgctggggcg tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg
       61 ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg
      121 cgtcgcatcg accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat
      181 agagttggtc aagaccaatg cggagcatat acgcccggag ccgcggcgat cctgcaagct
      241 ccggatgcct ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc
      301 agaagaagat gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa
      361 tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca
      421 cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg
      481 cgacggacgc actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag
      541 caatcgcgca tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg
      601 ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg gcctccgcga
      661 ccggctgcag aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg
      721 cacggcggga gatgcaatag gtcaggctct cgctgaattc cccaatgtca agcacttccg
      781 gaatcgggag cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat
      841 cggcgcagct atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac
      901 gagat
//
