LOCUS       sequence-142349-        9792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9792)
  AUTHORS   Graser S, Stierhof YD, Nigg EA
  TITLE     Cep68 and Cep215 (Cdk5rap2) are required for centrosome cohesion.
  JOURNAL   J Cell Sci. 2007 Dec 15;120(Pt 24):4321-31. Epub 2007 Nov 27.
  PUBMED    18042621
REFERENCE   2  (bases 1 to 9792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2007-12-15"; volume: "120(Pt 24)"; pages: "4321-31. 
            Epub 2007 Nov 27"
COMMENT     Sequence Label: pRcCMV Cep215 (Nigg CW493)
FEATURES             Location/Qualifiers
     source          1..9792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        68..371
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        372..575
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     526..546
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             692..721
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     RBS             1466..1477
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     polyA_signal    6587..6708
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6624..6643)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6678..6697
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6715..7170)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6852..6873)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7064..7083
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7197..7301
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7303..7660
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7303..7494
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7326..7346)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7511..7646
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7695..8489
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7695..8489
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7749..7768)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8359..8378
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8677..8696)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8721..8768
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9097..9685
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9586..9605
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
       61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      601 agcgctaccg gactcagatc tcgagctcaa gcttgcttac atttgcttct gacacaactg
      661 tgttcactag caacctcaaa cagacaccat ggagcagaag ctgatctccg aggaggacct
      721 gaacatgaat tcggccggcc tgaccatgat ggacttggtg ttggaagagg acgtcaccgt
      781 ccctgggacg ctcagcggct gcagtggcct tgttcccagt gtaccagatg acctggatgg
      841 catcaacccc aatgctgggt tgggaaatgg tctgctccca aatgtgtcag aagaaacagt
      901 gtctcccacc agagcacgga acatgaagga ctttgaaaat caaatcactg aattgaagaa
      961 agaaaacttt aacctaaagc tccgcatcta tttccttgag gaaagaatgc aacaggaatt
     1021 tcatggcccc actgaacata tctacaaaac taacattgag ctcaaggtgg aagtagaaag
     1081 tctgaagcgg gaactccagg agagagagca gctgctcatc aaagcctcca aagcagttga
     1141 gagcttagct gaagcaggtg gctctgaaat ccagcgggtg aaagaagatg ctcgaaagaa
     1201 ggtgcagcag gtggaagatc tcctaactaa aagaatactc cttttggaaa aggatgtgac
     1261 agccgcccag gcagaactgg aaaaggcctt tgcagggaca gagacggaga aggctcttcg
     1321 gttgcgtttg gaaagcaagc tttcagagat gaagaagatg cacgaggggg acttggcgat
     1381 ggctctggtc ctggatgaga aagacagact gattgaggag ttgaagctgt ctttgaagag
     1441 caaagaagct ttaattcagt gccttaaaga ggagaaatct cagatggcat gtcctgatga
     1501 gaatgtgtca tctggagagc tccgaggact ttgtgctgct ccaagggaag aaaaggagag
     1561 agaaactgag gctgcacaaa tggagcatca gaaggagaga aacagctttc aagagaggat
     1621 ccaggcactt gaagaggacc tgagagagaa ggaaagagaa attgctacag agaagaaaaa
     1681 tagtctaaag agggataaag ccattcaggg tttaaccatg gcattaaaat caaaggaaaa
     1741 aaaggttgaa gaacttaact ctgaaattga aaagctcagt gctgcctttg ctaaagccag
     1801 agaggcccta cagaaagcac agacccagga atttcagggg tctgaagact atgagactgc
     1861 tctatcagga aaggaagccc tttcggctgc gctgcgctca caaaacctca ccaagagtac
     1921 agagaaccac agactgcgta gaagcattaa gaagatcacc caggagctga gtgacttgca
     1981 gcaggagagg gagagactgg agaaggacct ggaggaagcc catcgagaga agagcaaagg
     2041 agactgcacc atccgtgatc ttagaaatga agttgaaaaa ttacgcaatg aagtgaatga
     2101 aagagagaaa gcaatggaaa atcgttacaa gagtcttctg agtgaaagca ataaaaaatt
     2161 gcacaatcaa gagcaagtga tcaaacatct aacagaaagt accaatcaga aggacgtgtt
     2221 gcttcagaaa ttcaatgaaa aagatttgga agtaatacag cagaactgct atttaatggc
     2281 tgcagaggat cttgagctca ggagtgaagg cttaataaca gaaaagtgct cttctcaaca
     2341 gccaccaggc agcaaaacca tcttctctaa ggaaaagaaa caatcatcag actatgaaga
     2401 gctgattcag gtcttaaaga aagagcagga catctatacc catctggtca aatctctgca
     2461 ggaatcagac agtatcaaca acctgcaggc tgagttaaac aagatttttg ccctgcggaa
     2521 gcaactggag caggatgtgc tttcatatca gaatttgcgg aagaccctcg aggagcagat
     2581 cagcgaaatt cggaggcggg aagaagaatc attttcactt tatagtgatc aaacatttta
     2641 tctaagtatt tgccttgaag aaaacaatcg gtttcaagtg gaacattttt ctcaagaaga
     2701 acttaagaaa aaggtcagtg accttataca gctagtgaag gagctgtata cagacaacca
     2761 gcacctgaag aaaaccattt ttgatctctc ctgcatgggt ttccagggaa atgggtttcc
     2821 agatagactt gcgtctacag aacaaacaga gcttctggct agcaaggagg acgaggacac
     2881 gatcaaaatt ggggaggatg acgagattaa tttcctgagt gaccagcatt tgcagcagag
     2941 taatgagatt atgaaagacc tttccaaagg aggctgcaaa aatggatact taaggcacac
     3001 ggagtctaag atttcagatt gtgatggggc ccacgcacct ggctgcctag aagaaggtgc
     3061 attcataaac ctgcttgccc ctttgttcaa tgagaaggcc acattattac tggaatccag
     3121 gccagacctt ctgaaagtgg tacgggaact gcttctggga caactattct tgacagagca
     3181 ggaagtttct ggagaacacc ttgatggtaa aactgagaag acacctaagc aaaaaggtga
     3241 acttgtacat tttgtccaaa ccaactcatt ttccaagcca catgatgaac tgaagttgtc
     3301 ttgtgaggcc cagctagtaa aggcaggcga agtgcccaag gtaggactga aagatgcctc
     3361 agtgcagact gtggccacgg agggcgacct gctgagattc aagcatgaag caacaagaga
     3421 ggcttgggaa gagaaaccga tcaacactgc actcagcgca gagcatcggc cagagaacct
     3481 gcacggggtg cctgggtggc aggctgccct cctttccctc cctggtatta ccaacagaga
     3541 ggctaagaag tcccgcttgc caatcctaat aaaaccatcc cggtcattag gaaatatgta
     3601 tcgtctccct gccacccagg aggtggtgac gcagctgcag agccagatct tggagctgca
     3661 gggggagctg aaggagttta aaacttgtaa taagcaactt caccaaaagt taattctggc
     3721 tgaagcagtg atggagggga ggccaacgcc cgacaaaacg ttgctgaatg ctcagccccc
     3781 tgtgggagca gcctaccagg acagcccagg agagcagaaa ggaattaaaa ccacatcttc
     3841 tgtctggaga gacaaggaaa tggacagtga tcagcaaaga agctacgaga ttgactctga
     3901 gatttgccca cctgatgacc ttgccagctt gccatcatgc aaagaaaatc ctgaagatgt
     3961 tctgagccca acttcagtag ctacttacct gagttccaag agtcagcctt ctgctaaagt
     4021 cagtgtgatg gggactgatc agtcagagag cattaatacc tcaaatgaga cagaatactt
     4081 aaaacagaaa atccatgact tggaaactga gctggaaggc taccagaatt tcatatttca
     4141 gcttcaaaag cactcccagt gcagtgaggc cataattaca gttttgtgtg ggacagaagg
     4201 ggcccaggat ggcttgagca agcccaagaa tggttctgat ggggaagaaa tgaccttttc
     4261 aagtttgcac caagtgcgat acgtgaaaca cgtgaaaatc ctcggtccgc tggccccaga
     4321 gatgattgac agcagggtgc tggagaacct caaacagcag ctggaggaac aggaatacaa
     4381 gctgcagaag gagcagaatt tgaacatgca acttttcagt gagatccata atctgcagaa
     4441 taagttcaga gatctctcac ctcccagata cgattcatta gttcagtccc aagccaggga
     4501 gctctccctt caacggcagc agattaagga tggccatggc atctgtgtca tctcccgtca
     4561 acacatgaac accatgatta aggcatttga ggagttgctg caggccagtg atgtggatta
     4621 ctgtgtggcc gagggtttcc aggaacagct gaatcaatgt gctgagctgc tggagaaatt
     4681 ggaaaagcta tttctcaacg gaaaatcagt tggagtggaa atgaacaccc agaatgaact
     4741 gatggagagg attgaggaag acaacttaac ctaccaacat cttctgcctg aatctcctga
     4801 gccttcagcc tctcatgcgc tctctgatta tgaaacatct gaaaagtcct tcttctcacg
     4861 agaccagaag caagataatg agacagagaa gacttcagtt atggtgaaca gtttttctca
     4921 agacttacta atggaacaca tacaggaaat tcgaactttg agaaagcgtt tagaagaatc
     4981 tattaaaaca aatgagaagc tacggaaaca gttggaacgg caaggatctg aatttgttca
     5041 aggttctaca agcatttttg cttctggttc agagcttcat agttctctaa catcagaaat
     5101 tcatttcttg aggaagcaga accaggccct caatgcaatg ctcattaaag gatccagaga
     5161 taaacagaag gagaatgaca aattacgaga gtccctctcc aggaagaccg tgagcctgga
     5221 gcaccttcag cgggagtatg ccagcgtgaa ggaagaaaat gaaaggctgc agaaagaagg
     5281 cagcgagaag gagagacaca accagcagct gatccaggag gtccgctgca gcggccagga
     5341 gctgagcagg gtgcaggagg agctgaagtt gaggcagcag ctgctctcac agaatgacaa
     5401 gctattgcag tctctccgag tggagctgaa ggcgtatgag aagctggatg aagagcacag
     5461 gagactgaga gaggcgtcgg gagaaggctg gaaggggcag gatcctttca gggacctgca
     5521 cagcctcctg atggagatcc aggctctgcg cttgcaacta gaaaggagca tcgaaaccag
     5581 cagcactctg cagagcaggc tcaaggaaca gctggcaagg ggggcagaga aggcacagga
     5641 aggagccctc actctggctg tccaagccgt gtccatccct gaggtgcccc ttcagcctga
     5701 caaacacgat ggtgacaaat atcccatgga aagtgataat tcatttgatc tgtttgattc
     5761 ctcccaggca gtgacaccaa aatcagtttc agagactcct ccactctctg ggaatgacac
     5821 ggactccctc tcctgcgaca gtggcagttc ggcaactagc actccgtgtg tgtcccgcct
     5881 ggtcactggc caccacctgt gggccagcaa gaatggccgc catgtcctgg gcctgattga
     5941 ggactatgag gccctgctca aacagatcag ccagggacag aggctccttg ctgaaatgga
     6001 cattcaaacc caagaggctc ccagctccac aagtcaagag ctgggaacaa agggtccaca
     6061 cccagcacca ctgagcaagt ttgtgagcag tgtgagcacg gccaagctga ccctggaaga
     6121 ggcctacagg cggctgaagc ttctctggag agtctcactc cccgaggatg gccagtgccc
     6181 ccttcactgt gagcagattg gagaaatgaa ggcagaggtc accaaactac ataaaaaatt
     6241 gtttgaacaa gaaaagaagt tgcaaaacac catgaagctt ttgcagctga gcaagcgcca
     6301 ggaaaaagtc atctttgatc aattggtcgt aacccacaaa atccttcgga aggccagagg
     6361 aaacctggag cttaggcctg ggggagccca tccaggaaca tgcagtccca gcagaccagg
     6421 ctccgcggcc gctgcagtcg acggtaccgc gggcccggga tccaccggat ctagataact
     6481 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     6541 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     6601 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     6661 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaac gcgtaaattg
     6721 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     6781 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     6841 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     6901 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     6961 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     7021 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     7081 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     7141 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg
     7201 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     7261 aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca
     7321 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     7381 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     7441 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     7501 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     7561 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     7621 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat
     7681 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     7741 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     7801 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     7861 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     7921 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     7981 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     8041 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     8101 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     8161 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     8221 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     8281 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     8341 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     8401 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     8461 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     8521 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     8581 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     8641 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc
     8701 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc
     8761 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
     8821 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
     8881 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg
     8941 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     9001 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     9061 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     9121 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     9181 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     9241 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     9301 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     9361 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     9421 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     9481 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     9541 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     9601 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     9661 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     9721 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     9781 ccgtattacc gc
//
