LOCUS       sequence-142351-         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses high specificity SpCas9. Px330-like plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Slaymaker IM, Gao L, Zetsche B, Scott DA, Yan WX, Zhang F
  TITLE     Rationally engineered Cas9 nucleases with improved specificity.
  JOURNAL   Science. 2015 Dec 1. pii: aad5227.
  PUBMED    26628643
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2015 Dec 1. pii: aad5227."
COMMENT     Sequence Label: eSpCas9(1.1)
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(217..292)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(311..559)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(369..388)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(539..559)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(701..720)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaaatactc cacccattga cgttcaatgg aaagtcccta ttggcgttac tattgacgtc
       61 aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg
      121 ggtacctcta gagccatttg tctgcagaat tggcgcacgc gctaaaaacg gactagcctt
      181 attttaactt gctatttcta gctctaaaac aaaaaagcac cgactcggtg ccactttttc
      241 aagttgataa cggactagcc ttattttaac ttgctatttc tagctctaaa acaggtcttc
      301 tcgaagaccc ggtgtttcgt cctttccaca agatatataa agccaagaaa tcgaaatact
      361 ttcaagttac ggtaagcata tgatagtcca ttttaaaaca taattttaaa actgcaaact
      421 acccaagaaa ttattacttt ctacgtcacg tattttgtac taatatcttt gtgtttacag
      481 tcaaattaat tccaattatc tctctaacag ccttgtatcg tatatgcaaa tatgaaggaa
      541 tcatgggaaa taggccctca catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      601 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
      661 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      721 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      781 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      841 tcggtgtagg tcgttcgctc ca
//
